Experimental demonstration of kinetic proofreading inherited in ligation-based information replication
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arXiv
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| Auteurs principaux: | , , |
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| Format: | Preprint |
| Publié: |
2025
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| _version_ | 1866912755257180160 |
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| author | Aoyanagi, Hiroyuki Magi, Yasuhiro Toyabe, Shoichi |
| author_facet | Aoyanagi, Hiroyuki Magi, Yasuhiro Toyabe, Shoichi |
| contents | We experimentally demonstrate that information replication by templated ligation of DNA strands inherits a kinetic proofreading mechanism and achieves significant error suppression through cascade replication. A simple simulation model derived from the experimental results shows that templated ligation has a significant advantage over replication by polymerization for error suppression of long strands. Specifically, longer chains show lower error rates, significantly distinct from the chain-growth polymerization where errors typically accumulate with chain length. This mechanism provides a plausible route for high-fidelity replication in prebiotic chemistry and illustrates how physical principles such as nonequilibrium kinetics and network architecture can drive reliable molecular information replication. The approach also offers new strategies for error suppression in biotechnology. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2505_08232 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Experimental demonstration of kinetic proofreading inherited in ligation-based information replication Aoyanagi, Hiroyuki Magi, Yasuhiro Toyabe, Shoichi Biological Physics Statistical Mechanics We experimentally demonstrate that information replication by templated ligation of DNA strands inherits a kinetic proofreading mechanism and achieves significant error suppression through cascade replication. A simple simulation model derived from the experimental results shows that templated ligation has a significant advantage over replication by polymerization for error suppression of long strands. Specifically, longer chains show lower error rates, significantly distinct from the chain-growth polymerization where errors typically accumulate with chain length. This mechanism provides a plausible route for high-fidelity replication in prebiotic chemistry and illustrates how physical principles such as nonequilibrium kinetics and network architecture can drive reliable molecular information replication. The approach also offers new strategies for error suppression in biotechnology. |
| title | Experimental demonstration of kinetic proofreading inherited in ligation-based information replication |
| topic | Biological Physics Statistical Mechanics |
| url | https://arxiv.org/abs/2505.08232 |