Physiological and Transcriptional Responses of Arabidopsis thaliana to Simulated Lunar and Martian Regolith Substrates
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| Format: | Preprint |
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2025
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| _version_ | 1866916862889033728 |
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| author | Buckner, A'nya Lang, Sarah Loureiro, Rafael |
| author_facet | Buckner, A'nya Lang, Sarah Loureiro, Rafael |
| contents | The integration of plant-based bioregenerative life support systems is a central objective in NASA's Moon to Mars strategy. Arabidopsis thaliana, a model organism with extensive genomic resources, serves as a key species to investigate plant resilience in extraterrestrial environments. We assessed the physiological and gene expression responses of A. thaliana (Col-0) grown in two off-world regolith simulants: LHS-2 (lunar highlands) and MGS-1 (Martian global). Plants exposed to these substrates exhibited significant reductions in root elongation, biomass, and chlorophyll content, along with elevated anthocyanin levels and transcriptional upregulation of stress-related genes including IRT1, PCS1, SOD1, and JAZ1. Evidence of jasmonic acid pathway activation and auxin signaling suppression suggests metal-induced hormonal misregulation. Our integrated analysis of morphological traits, pigment accumulation, and transcriptomic profiles reveals distinct mineral-specific stress responses, offering critical insights into substrate engineering strategies for future space agriculture. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2505_13583 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Physiological and Transcriptional Responses of Arabidopsis thaliana to Simulated Lunar and Martian Regolith Substrates Buckner, A'nya Lang, Sarah Loureiro, Rafael Quantitative Methods The integration of plant-based bioregenerative life support systems is a central objective in NASA's Moon to Mars strategy. Arabidopsis thaliana, a model organism with extensive genomic resources, serves as a key species to investigate plant resilience in extraterrestrial environments. We assessed the physiological and gene expression responses of A. thaliana (Col-0) grown in two off-world regolith simulants: LHS-2 (lunar highlands) and MGS-1 (Martian global). Plants exposed to these substrates exhibited significant reductions in root elongation, biomass, and chlorophyll content, along with elevated anthocyanin levels and transcriptional upregulation of stress-related genes including IRT1, PCS1, SOD1, and JAZ1. Evidence of jasmonic acid pathway activation and auxin signaling suppression suggests metal-induced hormonal misregulation. Our integrated analysis of morphological traits, pigment accumulation, and transcriptomic profiles reveals distinct mineral-specific stress responses, offering critical insights into substrate engineering strategies for future space agriculture. |
| title | Physiological and Transcriptional Responses of Arabidopsis thaliana to Simulated Lunar and Martian Regolith Substrates |
| topic | Quantitative Methods |
| url | https://arxiv.org/abs/2505.13583 |