Asymptotic Robustness in Biochemical Systems

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Main Authors: Hong, Hyukpyo, La Luz, Diego Rojas, Craciun, Gheorghe
Format: Preprint
Published: 2025
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author Hong, Hyukpyo
La Luz, Diego Rojas
Craciun, Gheorghe
author_facet Hong, Hyukpyo
La Luz, Diego Rojas
Craciun, Gheorghe
contents Living systems maintain stable internal states despite environmental fluctuations. Absolute concentration robustness (ACR) is a striking homeostatic phenomenon in which the steady-state concentration of a species remains invariant despite changes in total supply. Although experimental studies have reported approximate-but not exact-robustness in steady-state concentrations, such behavior has often been attributed to exact ACR motifs perturbed by measurement noise or minor reactions, rather than recognized as a structural property of a network itself. Here, we introduce a previously underappreciated phenomenon, namely asymptotic ACR (aACR): approximate robustness can emerge solely from the network structure, without requiring exact ACR motifs or negligible parameters. We find that aACR is more pervasive than classical ACR, as demonstrated in systems such as the Escherichia coli EnvZ-OmpR osmoregulation system and a futile cycle. Furthermore, we prove that such ubiquity stems solely from network structure without fine-tuning of kinetic parameters. The notion of aACR provides a rigorous and practical tool to analyze robust responses in broad biochemical systems.
format Preprint
id arxiv_https___arxiv_org_abs_2505_21900
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Asymptotic Robustness in Biochemical Systems
Hong, Hyukpyo
La Luz, Diego Rojas
Craciun, Gheorghe
Dynamical Systems
Quantitative Methods
37N25 (Primary) 34E18, 92B99 (Secondary)
Living systems maintain stable internal states despite environmental fluctuations. Absolute concentration robustness (ACR) is a striking homeostatic phenomenon in which the steady-state concentration of a species remains invariant despite changes in total supply. Although experimental studies have reported approximate-but not exact-robustness in steady-state concentrations, such behavior has often been attributed to exact ACR motifs perturbed by measurement noise or minor reactions, rather than recognized as a structural property of a network itself. Here, we introduce a previously underappreciated phenomenon, namely asymptotic ACR (aACR): approximate robustness can emerge solely from the network structure, without requiring exact ACR motifs or negligible parameters. We find that aACR is more pervasive than classical ACR, as demonstrated in systems such as the Escherichia coli EnvZ-OmpR osmoregulation system and a futile cycle. Furthermore, we prove that such ubiquity stems solely from network structure without fine-tuning of kinetic parameters. The notion of aACR provides a rigorous and practical tool to analyze robust responses in broad biochemical systems.
title Asymptotic Robustness in Biochemical Systems
topic Dynamical Systems
Quantitative Methods
37N25 (Primary) 34E18, 92B99 (Secondary)
url https://arxiv.org/abs/2505.21900