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| Autores principales: | , , |
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| Formato: | Preprint |
| Publicado: |
2025
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| Materias: | |
| Acceso en línea: | https://arxiv.org/abs/2506.05365 |
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| _version_ | 1866908395349475328 |
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| author | Moscatelli, J. Gonin, X Benoit Elias, F. |
| author_facet | Moscatelli, J. Gonin, X Benoit Elias, F. |
| contents | We introduce a device developed to perform a 3D tracking of passive or active particles under flow, confined in a medium of hundreds micrometers wide. Micro-objects are placed inside a vertical glass capillary and two mirrors are set behind it with a certain angle, making it possible to have the two reflections of the capillary on the same optical plane. A 3D reconstruction of the trajectories, captured with a single camera, is carried out along the vertical axis with a micrometer-scale precision. To investigate the interaction between the shear, the role of the gravity field, and motile microorganism, we track a model puller-type microalgae, Chlamydomonas reinhardtii under a Poiseuille flow, using first its natural fluorescence and then a bright-field imaging. Understanding how confinement influences motility is crucial, and we show that this 3D tracking setup enables a full description of interactions between a motile organism and a solid border. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2506_05365 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | 3D tracking of Plankton with single-camera stereoscopy Moscatelli, J. Gonin, X Benoit Elias, F. Soft Condensed Matter We introduce a device developed to perform a 3D tracking of passive or active particles under flow, confined in a medium of hundreds micrometers wide. Micro-objects are placed inside a vertical glass capillary and two mirrors are set behind it with a certain angle, making it possible to have the two reflections of the capillary on the same optical plane. A 3D reconstruction of the trajectories, captured with a single camera, is carried out along the vertical axis with a micrometer-scale precision. To investigate the interaction between the shear, the role of the gravity field, and motile microorganism, we track a model puller-type microalgae, Chlamydomonas reinhardtii under a Poiseuille flow, using first its natural fluorescence and then a bright-field imaging. Understanding how confinement influences motility is crucial, and we show that this 3D tracking setup enables a full description of interactions between a motile organism and a solid border. |
| title | 3D tracking of Plankton with single-camera stereoscopy |
| topic | Soft Condensed Matter |
| url | https://arxiv.org/abs/2506.05365 |