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Autori principali: Jain, Garima, Gupta, Ravi Kant, Jain, Priyansh, Patil, Abhijeet, Sekhar, Ardhendu, Smeeta, Gajendra, Pati, Sanghamitra, Sethi, Amit
Natura: Preprint
Pubblicazione: 2025
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Accesso online:https://arxiv.org/abs/2506.12798
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author Jain, Garima
Gupta, Ravi Kant
Jain, Priyansh
Patil, Abhijeet
Sekhar, Ardhendu
Smeeta, Gajendra
Pati, Sanghamitra
Sethi, Amit
author_facet Jain, Garima
Gupta, Ravi Kant
Jain, Priyansh
Patil, Abhijeet
Sekhar, Ardhendu
Smeeta, Gajendra
Pati, Sanghamitra
Sethi, Amit
contents In this study, we propose a robust methodology for identification of myeloid blasts followed by prediction of genetic mutation in single-cell images of blasts, tackling challenges associated with label accuracy and data noise. We trained an initial binary classifier to distinguish between leukemic (blasts) and non-leukemic cells images, achieving 90 percent accuracy. To evaluate the models generalization, we applied this model to a separate large unlabeled dataset and validated the predictions with two haemato-pathologists, finding an approximate error rate of 20 percent in the leukemic and non-leukemic labels. Assuming this level of label noise, we further trained a four-class model on images predicted as blasts to classify specific mutations. The mutation labels were known for only a bag of cell images extracted from a single slide. Despite the tumor label noise, our mutation classification model achieved 85 percent accuracy across four mutation classes, demonstrating resilience to label inconsistencies. This study highlights the capability of machine learning models to work with noisy labels effectively while providing accurate, clinically relevant mutation predictions, which is promising for diagnostic applications in areas such as haemato-pathology.
format Preprint
id arxiv_https___arxiv_org_abs_2506_12798
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Predicting Genetic Mutations from Single-Cell Bone Marrow Images in Acute Myeloid Leukemia Using Noise-Robust Deep Learning Models
Jain, Garima
Gupta, Ravi Kant
Jain, Priyansh
Patil, Abhijeet
Sekhar, Ardhendu
Smeeta, Gajendra
Pati, Sanghamitra
Sethi, Amit
Image and Video Processing
Computer Vision and Pattern Recognition
In this study, we propose a robust methodology for identification of myeloid blasts followed by prediction of genetic mutation in single-cell images of blasts, tackling challenges associated with label accuracy and data noise. We trained an initial binary classifier to distinguish between leukemic (blasts) and non-leukemic cells images, achieving 90 percent accuracy. To evaluate the models generalization, we applied this model to a separate large unlabeled dataset and validated the predictions with two haemato-pathologists, finding an approximate error rate of 20 percent in the leukemic and non-leukemic labels. Assuming this level of label noise, we further trained a four-class model on images predicted as blasts to classify specific mutations. The mutation labels were known for only a bag of cell images extracted from a single slide. Despite the tumor label noise, our mutation classification model achieved 85 percent accuracy across four mutation classes, demonstrating resilience to label inconsistencies. This study highlights the capability of machine learning models to work with noisy labels effectively while providing accurate, clinically relevant mutation predictions, which is promising for diagnostic applications in areas such as haemato-pathology.
title Predicting Genetic Mutations from Single-Cell Bone Marrow Images in Acute Myeloid Leukemia Using Noise-Robust Deep Learning Models
topic Image and Video Processing
Computer Vision and Pattern Recognition
url https://arxiv.org/abs/2506.12798