SPADE: Spatial Transcriptomics and Pathology Alignment Using a Mixture of Data Experts for an Expressive Latent Space

Fuente: arXiv
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Main Authors: Redekop, Ekaterina, Pleasure, Mara, Wang, Zichen, Flores, Kimberly, Sisk, Anthony, Speier, William, Arnold, Corey W.
Format: Preprint
Published: 2025
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author Redekop, Ekaterina
Pleasure, Mara
Wang, Zichen
Flores, Kimberly
Sisk, Anthony
Speier, William
Arnold, Corey W.
author_facet Redekop, Ekaterina
Pleasure, Mara
Wang, Zichen
Flores, Kimberly
Sisk, Anthony
Speier, William
Arnold, Corey W.
contents The rapid growth of digital pathology and advances in self-supervised deep learning have enabled the development of foundational models for various pathology tasks across diverse diseases. While multimodal approaches integrating diverse data sources have emerged, a critical gap remains in the comprehensive integration of whole-slide images (WSIs) with spatial transcriptomics (ST), which is crucial for capturing critical molecular heterogeneity beyond standard hematoxylin & eosin (H&E) staining. We introduce SPADE, a foundation model that integrates histopathology with ST data to guide image representation learning within a unified framework, in effect creating an ST-informed latent space. SPADE leverages a mixture-of-data experts technique, where experts are created via two-stage imaging feature-space clustering using contrastive learning to learn representations of co-registered WSI patches and gene expression profiles. Pre-trained on the comprehensive HEST-1k dataset, SPADE is evaluated on 20 downstream tasks, demonstrating significantly superior few-shot performance compared to baseline models, highlighting the benefits of integrating morphological and molecular information into one latent space. Code and pretrained weights are available at https://github.com/uclabair/SPADE.
format Preprint
id arxiv_https___arxiv_org_abs_2506_21857
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle SPADE: Spatial Transcriptomics and Pathology Alignment Using a Mixture of Data Experts for an Expressive Latent Space
Redekop, Ekaterina
Pleasure, Mara
Wang, Zichen
Flores, Kimberly
Sisk, Anthony
Speier, William
Arnold, Corey W.
Computer Vision and Pattern Recognition
Artificial Intelligence
Machine Learning
The rapid growth of digital pathology and advances in self-supervised deep learning have enabled the development of foundational models for various pathology tasks across diverse diseases. While multimodal approaches integrating diverse data sources have emerged, a critical gap remains in the comprehensive integration of whole-slide images (WSIs) with spatial transcriptomics (ST), which is crucial for capturing critical molecular heterogeneity beyond standard hematoxylin & eosin (H&E) staining. We introduce SPADE, a foundation model that integrates histopathology with ST data to guide image representation learning within a unified framework, in effect creating an ST-informed latent space. SPADE leverages a mixture-of-data experts technique, where experts are created via two-stage imaging feature-space clustering using contrastive learning to learn representations of co-registered WSI patches and gene expression profiles. Pre-trained on the comprehensive HEST-1k dataset, SPADE is evaluated on 20 downstream tasks, demonstrating significantly superior few-shot performance compared to baseline models, highlighting the benefits of integrating morphological and molecular information into one latent space. Code and pretrained weights are available at https://github.com/uclabair/SPADE.
title SPADE: Spatial Transcriptomics and Pathology Alignment Using a Mixture of Data Experts for an Expressive Latent Space
topic Computer Vision and Pattern Recognition
Artificial Intelligence
Machine Learning
url https://arxiv.org/abs/2506.21857