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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Preprint |
| Published: |
2025
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| Subjects: | |
| Online Access: | https://arxiv.org/abs/2507.03186 |
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| _version_ | 1866912465211621376 |
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| author | Alarcón, Marta Girona Kuo, Willy Humbel, Mattia Tanner, Christine Fardin, Luca Bausch, Britta Decker, Yann Spera, Irene Rodgers, Griffin Deyhle, Hans Bravin, Alberto Hoshino, Masato Panahifar, Arash Uesugi, Kentaro Gasilov, Sergei Pleskač, Petr Zhang, Yuansheng de Zélicourt, Diane Brenna, Amandine Hamid, Ahmad Kamal Khamesi, Pooya Razzaghi Engelhardt, Britta Proulx, Steven T. Müller, Bert Kurtcuoglu, Vartan |
| author_facet | Alarcón, Marta Girona Kuo, Willy Humbel, Mattia Tanner, Christine Fardin, Luca Bausch, Britta Decker, Yann Spera, Irene Rodgers, Griffin Deyhle, Hans Bravin, Alberto Hoshino, Masato Panahifar, Arash Uesugi, Kentaro Gasilov, Sergei Pleskač, Petr Zhang, Yuansheng de Zélicourt, Diane Brenna, Amandine Hamid, Ahmad Kamal Khamesi, Pooya Razzaghi Engelhardt, Britta Proulx, Steven T. Müller, Bert Kurtcuoglu, Vartan |
| contents | Current approaches to in vivo imaging of the mouse central nervous system (CNS) do not offer a combination of micrometer resolution and a whole-brain field of view. To address this limitation, we introduce an approach based on synchrotron radiation-based hard X-ray micro computed tomography (SR$μ$CT). We performed intravital SR$μ$CT acquisitions of mouse CNS fluid spaces at three synchrotron radiation facilities. Imaging was conducted on both anesthetized free-breathing and ventilated animals, with and without retrospective cardiac gating. We achieved whole-brain imaging at 6.3 $μ$m uniform voxel size, observed the distribution of cerebrospinal fluid (CSF) contrast agent over time and quantified choroid plexus movement. SR$μ$CT bridges the gap between multiphoton microscopy and magnetic resonance imaging, offering dynamic imaging with micrometer-scale resolution and whole-organ field of view. Intravital SR$μ$CT will play a crucial role in validating and integrating hypotheses on CSF dynamics and solute transport by providing unique data that cannot be acquired otherwise. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2507_03186 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | In vivo imaging of central nervous system fluid spaces using synchrotron radiation-based micro computed tomography Alarcón, Marta Girona Kuo, Willy Humbel, Mattia Tanner, Christine Fardin, Luca Bausch, Britta Decker, Yann Spera, Irene Rodgers, Griffin Deyhle, Hans Bravin, Alberto Hoshino, Masato Panahifar, Arash Uesugi, Kentaro Gasilov, Sergei Pleskač, Petr Zhang, Yuansheng de Zélicourt, Diane Brenna, Amandine Hamid, Ahmad Kamal Khamesi, Pooya Razzaghi Engelhardt, Britta Proulx, Steven T. Müller, Bert Kurtcuoglu, Vartan Medical Physics Current approaches to in vivo imaging of the mouse central nervous system (CNS) do not offer a combination of micrometer resolution and a whole-brain field of view. To address this limitation, we introduce an approach based on synchrotron radiation-based hard X-ray micro computed tomography (SR$μ$CT). We performed intravital SR$μ$CT acquisitions of mouse CNS fluid spaces at three synchrotron radiation facilities. Imaging was conducted on both anesthetized free-breathing and ventilated animals, with and without retrospective cardiac gating. We achieved whole-brain imaging at 6.3 $μ$m uniform voxel size, observed the distribution of cerebrospinal fluid (CSF) contrast agent over time and quantified choroid plexus movement. SR$μ$CT bridges the gap between multiphoton microscopy and magnetic resonance imaging, offering dynamic imaging with micrometer-scale resolution and whole-organ field of view. Intravital SR$μ$CT will play a crucial role in validating and integrating hypotheses on CSF dynamics and solute transport by providing unique data that cannot be acquired otherwise. |
| title | In vivo imaging of central nervous system fluid spaces using synchrotron radiation-based micro computed tomography |
| topic | Medical Physics |
| url | https://arxiv.org/abs/2507.03186 |