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Bibliographic Details
Main Authors: Alarcón, Marta Girona, Kuo, Willy, Humbel, Mattia, Tanner, Christine, Fardin, Luca, Bausch, Britta, Decker, Yann, Spera, Irene, Rodgers, Griffin, Deyhle, Hans, Bravin, Alberto, Hoshino, Masato, Panahifar, Arash, Uesugi, Kentaro, Gasilov, Sergei, Pleskač, Petr, Zhang, Yuansheng, de Zélicourt, Diane, Brenna, Amandine, Hamid, Ahmad Kamal, Khamesi, Pooya Razzaghi, Engelhardt, Britta, Proulx, Steven T., Müller, Bert, Kurtcuoglu, Vartan
Format: Preprint
Published: 2025
Subjects:
Medical Physics
Online Access:https://arxiv.org/abs/2507.03186
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Table of Contents:
  • Current approaches to in vivo imaging of the mouse central nervous system (CNS) do not offer a combination of micrometer resolution and a whole-brain field of view. To address this limitation, we introduce an approach based on synchrotron radiation-based hard X-ray micro computed tomography (SR$μ$CT). We performed intravital SR$μ$CT acquisitions of mouse CNS fluid spaces at three synchrotron radiation facilities. Imaging was conducted on both anesthetized free-breathing and ventilated animals, with and without retrospective cardiac gating. We achieved whole-brain imaging at 6.3 $μ$m uniform voxel size, observed the distribution of cerebrospinal fluid (CSF) contrast agent over time and quantified choroid plexus movement. SR$μ$CT bridges the gap between multiphoton microscopy and magnetic resonance imaging, offering dynamic imaging with micrometer-scale resolution and whole-organ field of view. Intravital SR$μ$CT will play a crucial role in validating and integrating hypotheses on CSF dynamics and solute transport by providing unique data that cannot be acquired otherwise.

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