Digital defocus aberration interference for automated optical microscopy
Fuente:
arXiv
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Preprint |
| Published: |
2025
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866911457648574464 |
|---|---|
| author | Zhou, Haowen Zhao, Shi Fan, Yujie Dong, Zhenyu Zhang, Oumeng Gradinaru, Viviana Yang, Changhuei |
| author_facet | Zhou, Haowen Zhao, Shi Fan, Yujie Dong, Zhenyu Zhang, Oumeng Gradinaru, Viviana Yang, Changhuei |
| contents | Automation in optical microscopy is critical for enabling high-throughput imaging across a wide range of biomedical applications. Among the essential components of automated systems, robust autofocusing plays a pivotal role in maintaining image quality for both single-plane and volumetric imaging. However, conventional autofocusing methods often struggle with implementation complexity, limited generalizability across sample types, incompatibility with thick specimens, and slow feedback. We observed that the digitally summed Fourier spectrum of two images acquired from two-angle illumination exhibits interference-like fringe modulation when the sample is defocused. These digital fringes correlate directly with defocus through a physics-based relation. Based on this principle, we developed an automatic, efficient, and generalizable defocus detection method termed digital defocus aberration interference (DAbI). Implemented with a simple two-LED setup, DAbI can quantify the defocus distance over a range of 443 times the depth-of-field (DoF) for thin samples and 296 times for thick specimens. It can additionally extend the natural DoF of the imaging system by 20 folds when integrated with complex-field imaging. We demonstrated the versatile applications of DAbI on brightfield, complex-field, refractive index, confocal, and widefield fluorescence imaging, establishing it as a promising solution for automated, high-throughput optical microscopy. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2507_10867 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Digital defocus aberration interference for automated optical microscopy Zhou, Haowen Zhao, Shi Fan, Yujie Dong, Zhenyu Zhang, Oumeng Gradinaru, Viviana Yang, Changhuei Optics Automation in optical microscopy is critical for enabling high-throughput imaging across a wide range of biomedical applications. Among the essential components of automated systems, robust autofocusing plays a pivotal role in maintaining image quality for both single-plane and volumetric imaging. However, conventional autofocusing methods often struggle with implementation complexity, limited generalizability across sample types, incompatibility with thick specimens, and slow feedback. We observed that the digitally summed Fourier spectrum of two images acquired from two-angle illumination exhibits interference-like fringe modulation when the sample is defocused. These digital fringes correlate directly with defocus through a physics-based relation. Based on this principle, we developed an automatic, efficient, and generalizable defocus detection method termed digital defocus aberration interference (DAbI). Implemented with a simple two-LED setup, DAbI can quantify the defocus distance over a range of 443 times the depth-of-field (DoF) for thin samples and 296 times for thick specimens. It can additionally extend the natural DoF of the imaging system by 20 folds when integrated with complex-field imaging. We demonstrated the versatile applications of DAbI on brightfield, complex-field, refractive index, confocal, and widefield fluorescence imaging, establishing it as a promising solution for automated, high-throughput optical microscopy. |
| title | Digital defocus aberration interference for automated optical microscopy |
| topic | Optics |
| url | https://arxiv.org/abs/2507.10867 |