SAMPLE -- Stratospheric Altitude Microbiology Probe for Life Existence -- A Method of Collection of Stratospheric Samples Using Balloon-Borne Payload System

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Main Authors: Safonova, Margarita, P, Bharat Chandra, Nair, Binukumar G., Datey, Akshay, Chakravortty, Dipshikha, Prakash, Ajin, Babu, Mahesh, Ghatul, Shubham, Jain, Shubhangi, Mohan, Rekhesh, Murthy, Jayant
Format: Preprint
Published: 2025
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author Safonova, Margarita
P, Bharat Chandra
Nair, Binukumar G.
Datey, Akshay
Chakravortty, Dipshikha
Prakash, Ajin
Babu, Mahesh
Ghatul, Shubham
Jain, Shubhangi
Mohan, Rekhesh
Murthy, Jayant
author_facet Safonova, Margarita
P, Bharat Chandra
Nair, Binukumar G.
Datey, Akshay
Chakravortty, Dipshikha
Prakash, Ajin
Babu, Mahesh
Ghatul, Shubham
Jain, Shubhangi
Mohan, Rekhesh
Murthy, Jayant
contents The Earth possesses many environmental extremes that mimic conditions on extraterrestrial worlds. The stratosphere at 30-40 km altitude closely resembles the surface of Mars in terms of pressure, temperature, and radiation levels (UV, proton, and Galactic cosmic rays). While microbial life in the troposphere is well documented, the true upper limit of Earth's biosphere remains unclear. The stratosphere offers a promising environment to explore microbial survival in such extreme conditions. Despite its significance to astrobiology, this region remains largely unexplored due to difficulties in access and avoiding contamination. To address this, we have developed SAMPLE (Stratospheric Altitude Microbiology Probe for Life Existence), a balloon-borne payload designed to collect dust samples from the stratosphere and return them in conditions suitable for lab analysis. The entire system is novel and designed in-house, with weight- and stress-optimized components. The main payload includes three pre-sterilized sampling trays and a controller that determines altitude and governs tray operation. One tray will remain closed during flight (airborne control) and another on the ground (cleanroom control) to monitor contamination. Additional systems include environmental sensors, GPS trackers, cameras, and a Flight Termination Unit (FTU) to end the mission once sampling is complete. A parachute ensures the safe recovery of the payload. Upon retrieving the payload, the sampling trays (including controls) will be sent to a suitable laboratory where the samples will be examined for the presence and nature of collected material.
format Preprint
id arxiv_https___arxiv_org_abs_2507_11581
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle SAMPLE -- Stratospheric Altitude Microbiology Probe for Life Existence -- A Method of Collection of Stratospheric Samples Using Balloon-Borne Payload System
Safonova, Margarita
P, Bharat Chandra
Nair, Binukumar G.
Datey, Akshay
Chakravortty, Dipshikha
Prakash, Ajin
Babu, Mahesh
Ghatul, Shubham
Jain, Shubhangi
Mohan, Rekhesh
Murthy, Jayant
Instrumentation and Methods for Astrophysics
Earth and Planetary Astrophysics
The Earth possesses many environmental extremes that mimic conditions on extraterrestrial worlds. The stratosphere at 30-40 km altitude closely resembles the surface of Mars in terms of pressure, temperature, and radiation levels (UV, proton, and Galactic cosmic rays). While microbial life in the troposphere is well documented, the true upper limit of Earth's biosphere remains unclear. The stratosphere offers a promising environment to explore microbial survival in such extreme conditions. Despite its significance to astrobiology, this region remains largely unexplored due to difficulties in access and avoiding contamination. To address this, we have developed SAMPLE (Stratospheric Altitude Microbiology Probe for Life Existence), a balloon-borne payload designed to collect dust samples from the stratosphere and return them in conditions suitable for lab analysis. The entire system is novel and designed in-house, with weight- and stress-optimized components. The main payload includes three pre-sterilized sampling trays and a controller that determines altitude and governs tray operation. One tray will remain closed during flight (airborne control) and another on the ground (cleanroom control) to monitor contamination. Additional systems include environmental sensors, GPS trackers, cameras, and a Flight Termination Unit (FTU) to end the mission once sampling is complete. A parachute ensures the safe recovery of the payload. Upon retrieving the payload, the sampling trays (including controls) will be sent to a suitable laboratory where the samples will be examined for the presence and nature of collected material.
title SAMPLE -- Stratospheric Altitude Microbiology Probe for Life Existence -- A Method of Collection of Stratospheric Samples Using Balloon-Borne Payload System
topic Instrumentation and Methods for Astrophysics
Earth and Planetary Astrophysics
url https://arxiv.org/abs/2507.11581