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Dettagli Bibliografici
Autori principali: Zhuang, Zhenfeng, Cen, Min, Jiang, Lei, Peng, Qiong, Hu, Yihuang, Zhou, Hong-Yu, Wang, Liansheng
Natura: Preprint
Pubblicazione: 2025
Soggetti:
Accesso online:https://arxiv.org/abs/2508.02423
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Sommario:
  • Histopathological analysis has been transformed by serial section-based methods, advancing beyond traditional 2D histology to enable volumetric and microstructural insights in oncology and inflammatory disease diagnostics. This review outlines key developments in specimen preparation and high-throughput imaging that support these innovations. Computational workflows are categorized into multimodal image co-registration, 3D histoarchitecture reconstruction, multiplexed immunohistochemical correlation, and cross-scale data fusion. These approaches exploit serial section-derived spatial concordance to enhance resolution in microenvironmental and molecular profiling. Despite progress, challenges remain in harmonizing heterogeneous datasets, optimizing large-scale registration, and ensuring interpretability. Future directions include spatial transcriptomics, and applications in developmental biology and neuroscience in AI integration, establishing serial section analytics as central to precision histopathology.