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| Main Authors: | , |
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| Format: | Preprint |
| Published: |
2025
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| Subjects: | |
| Online Access: | https://arxiv.org/abs/2508.13668 |
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| _version_ | 1866914342728892416 |
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| author | Xu, Lance W. Q. Pressé, Steve |
| author_facet | Xu, Lance W. Q. Pressé, Steve |
| contents | Tracking single fluorescent molecules has offered resolution into dynamic molecular processes at the single-molecule level. This perspective traces the evolution of single-molecule tracking, highlighting key developments across various methodological branches within fluorescence microscopy. We compare the strengths and limitations of each approach, ranging from conventional widefield offline tracking to real-time confocal tracking. In the final section, we explore emerging efforts to advance physics-inspired tracking techniques, a possibility for parallelization and artificial intelligence, and discuss challenges and opportunities they present toward achieving higher spatiotemporal resolution and greater computational and data efficiency in next-generation single-molecule studies. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2508_13668 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Perspective: An outlook on fluorescence tracking Xu, Lance W. Q. Pressé, Steve Biological Physics Computation Tracking single fluorescent molecules has offered resolution into dynamic molecular processes at the single-molecule level. This perspective traces the evolution of single-molecule tracking, highlighting key developments across various methodological branches within fluorescence microscopy. We compare the strengths and limitations of each approach, ranging from conventional widefield offline tracking to real-time confocal tracking. In the final section, we explore emerging efforts to advance physics-inspired tracking techniques, a possibility for parallelization and artificial intelligence, and discuss challenges and opportunities they present toward achieving higher spatiotemporal resolution and greater computational and data efficiency in next-generation single-molecule studies. |
| title | Perspective: An outlook on fluorescence tracking |
| topic | Biological Physics Computation |
| url | https://arxiv.org/abs/2508.13668 |