Ultrasensitive Label-free Detection of Human CEACAM5 using a WGM Resonator Based on Thin-walled Capillary
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arXiv
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| Format: | Preprint |
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2025
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| author | Cui, Xiaolu Ma, Jiyang Wu, Hao Zhao, Shuoying Peng, Xubiao Li, Chuyu Ming, Ruiqi Zhao, Qing |
| author_facet | Cui, Xiaolu Ma, Jiyang Wu, Hao Zhao, Shuoying Peng, Xubiao Li, Chuyu Ming, Ruiqi Zhao, Qing |
| contents | Whispering gallery mode (WGM) laser sensors, utilizing the interaction between the in-plane evanescent field and the surface vicinity, provide enhanced sensitivity in label-free sensing for bioanalysis and disease screening. However, the unavoidably excited spiral modes resulting from the weak axial confinement and their sensing potential were overlooked. In this study, a microfluidic biosensor using the localized conical modes of an active resonator based on the thin-walled capillaries was developed, demonstrating ultrasensitive refractive index and biomolecule detection capabilities. This sensor provides nonspecific detection of bovine serum albumin (BSA) and specific detection of Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) with ultra-low detection limits, large sensing range, rapid response, and a cost-effective design, making it a promising candidate for industrial-scale production. The theoretical detection limit for CEACAM5 is as low as 0.38ag/mL (5zM), and the sensitivity in the linear region reaches 0.25nm/(ag/mL). These results are approximately an order of magnitude higher in sensitivity than currently reported active WGM biosensors, demonstrating enormous detection potential for biomarkers. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2508_16058 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Ultrasensitive Label-free Detection of Human CEACAM5 using a WGM Resonator Based on Thin-walled Capillary Cui, Xiaolu Ma, Jiyang Wu, Hao Zhao, Shuoying Peng, Xubiao Li, Chuyu Ming, Ruiqi Zhao, Qing Optics Whispering gallery mode (WGM) laser sensors, utilizing the interaction between the in-plane evanescent field and the surface vicinity, provide enhanced sensitivity in label-free sensing for bioanalysis and disease screening. However, the unavoidably excited spiral modes resulting from the weak axial confinement and their sensing potential were overlooked. In this study, a microfluidic biosensor using the localized conical modes of an active resonator based on the thin-walled capillaries was developed, demonstrating ultrasensitive refractive index and biomolecule detection capabilities. This sensor provides nonspecific detection of bovine serum albumin (BSA) and specific detection of Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) with ultra-low detection limits, large sensing range, rapid response, and a cost-effective design, making it a promising candidate for industrial-scale production. The theoretical detection limit for CEACAM5 is as low as 0.38ag/mL (5zM), and the sensitivity in the linear region reaches 0.25nm/(ag/mL). These results are approximately an order of magnitude higher in sensitivity than currently reported active WGM biosensors, demonstrating enormous detection potential for biomarkers. |
| title | Ultrasensitive Label-free Detection of Human CEACAM5 using a WGM Resonator Based on Thin-walled Capillary |
| topic | Optics |
| url | https://arxiv.org/abs/2508.16058 |