Performance Validation of Coded Wavefront Sensing for Quantitative Phase Imaging of Static and Dynamic Specimens Using Digital Holographic Microscopy

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Hauptverfasser: Kazim, Syed Muhammad, Strasser, Franziska, Løvmo, Mia Kvåle, Nehrych, Andrii, Moser, Simon, Ziemczonok, Michał, Heidrich, Wolfgang, Ihrke, Ivo, Ritsch-Marte, Monika
Format: Preprint
Veröffentlicht: 2025
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author Kazim, Syed Muhammad
Strasser, Franziska
Løvmo, Mia Kvåle
Nehrych, Andrii
Moser, Simon
Ziemczonok, Michał
Heidrich, Wolfgang
Ihrke, Ivo
Ritsch-Marte, Monika
author_facet Kazim, Syed Muhammad
Strasser, Franziska
Løvmo, Mia Kvåle
Nehrych, Andrii
Moser, Simon
Ziemczonok, Michał
Heidrich, Wolfgang
Ihrke, Ivo
Ritsch-Marte, Monika
contents Coded wavefront sensing (Coded-WFS) is a snapshot quantitative phase imaging (QPI) technique that has been shown to successfully leverage the memory effect to retrieve the phase of biological specimens. In this paper, we perform QPI on static silica beads and dynamic HEK cells using Coded-WFS. The accuracy of the retrieved phase map is validated using digital holographic microscopy (DHM) for the same specimens. We report comparisons of simultaneous bright-field intensity and optical path delay.
format Preprint
id arxiv_https___arxiv_org_abs_2508_17143
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Performance Validation of Coded Wavefront Sensing for Quantitative Phase Imaging of Static and Dynamic Specimens Using Digital Holographic Microscopy
Kazim, Syed Muhammad
Strasser, Franziska
Løvmo, Mia Kvåle
Nehrych, Andrii
Moser, Simon
Ziemczonok, Michał
Heidrich, Wolfgang
Ihrke, Ivo
Ritsch-Marte, Monika
Optics
Signal Processing
Coded wavefront sensing (Coded-WFS) is a snapshot quantitative phase imaging (QPI) technique that has been shown to successfully leverage the memory effect to retrieve the phase of biological specimens. In this paper, we perform QPI on static silica beads and dynamic HEK cells using Coded-WFS. The accuracy of the retrieved phase map is validated using digital holographic microscopy (DHM) for the same specimens. We report comparisons of simultaneous bright-field intensity and optical path delay.
title Performance Validation of Coded Wavefront Sensing for Quantitative Phase Imaging of Static and Dynamic Specimens Using Digital Holographic Microscopy
topic Optics
Signal Processing
url https://arxiv.org/abs/2508.17143