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Main Authors: Zhang, Jing, Tao, Siying, Li, Jiao, Wang, Tianhe, Wu, Junchen, Hao, Ruqian, Du, Xiaohui, Tan, Ruirong, Li, Rui
Format: Preprint
Published: 2025
Subjects:
Online Access:https://arxiv.org/abs/2509.03221
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author Zhang, Jing
Tao, Siying
Li, Jiao
Wang, Tianhe
Wu, Junchen
Hao, Ruqian
Du, Xiaohui
Tan, Ruirong
Li, Rui
author_facet Zhang, Jing
Tao, Siying
Li, Jiao
Wang, Tianhe
Wu, Junchen
Hao, Ruqian
Du, Xiaohui
Tan, Ruirong
Li, Rui
contents Organoids replicate organ structure and function, playing a crucial role in fields such as tumor treatment and drug screening. Their shape and size can indicate their developmental status, but traditional fluorescence labeling methods risk compromising their structure. Therefore, this paper proposes an automated, non-destructive approach to organoid segmentation and tracking. We introduced the LGBP-OrgaNet, a deep learning-based system proficient in accurately segmenting, tracking, and quantifying organoids. The model leverages complementary information extracted from CNN and Transformer modules and introduces the innovative feature fusion module, Learnable Gaussian Band Pass Fusion, to merge data from two branches. Additionally, in the decoder, the model proposes a Bidirectional Cross Fusion Block to fuse multi-scale features, and finally completes the decoding through progressive concatenation and upsampling. SROrga demonstrates satisfactory segmentation accuracy and robustness on organoids segmentation datasets, providing a potent tool for organoid research.
format Preprint
id arxiv_https___arxiv_org_abs_2509_03221
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle LGBP-OrgaNet: Learnable Gaussian Band Pass Fusion of CNN and Transformer Features for Robust Organoid Segmentation and Tracking
Zhang, Jing
Tao, Siying
Li, Jiao
Wang, Tianhe
Wu, Junchen
Hao, Ruqian
Du, Xiaohui
Tan, Ruirong
Li, Rui
Computer Vision and Pattern Recognition
Artificial Intelligence
Organoids replicate organ structure and function, playing a crucial role in fields such as tumor treatment and drug screening. Their shape and size can indicate their developmental status, but traditional fluorescence labeling methods risk compromising their structure. Therefore, this paper proposes an automated, non-destructive approach to organoid segmentation and tracking. We introduced the LGBP-OrgaNet, a deep learning-based system proficient in accurately segmenting, tracking, and quantifying organoids. The model leverages complementary information extracted from CNN and Transformer modules and introduces the innovative feature fusion module, Learnable Gaussian Band Pass Fusion, to merge data from two branches. Additionally, in the decoder, the model proposes a Bidirectional Cross Fusion Block to fuse multi-scale features, and finally completes the decoding through progressive concatenation and upsampling. SROrga demonstrates satisfactory segmentation accuracy and robustness on organoids segmentation datasets, providing a potent tool for organoid research.
title LGBP-OrgaNet: Learnable Gaussian Band Pass Fusion of CNN and Transformer Features for Robust Organoid Segmentation and Tracking
topic Computer Vision and Pattern Recognition
Artificial Intelligence
url https://arxiv.org/abs/2509.03221