Control of lumen morphology by lateral and basal cell surfaces
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arXiv
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| Auteurs principaux: | , , , |
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| Format: | Preprint |
| Publié: |
2025
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| _version_ | 1866911566095450112 |
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| author | Ray, Chandraniva Guha Mukenhirn, Markus Honigmann, Alf Haas, Pierre A. |
| author_facet | Ray, Chandraniva Guha Mukenhirn, Markus Honigmann, Alf Haas, Pierre A. |
| contents | Across development, the morphology of fluid-filled lumina enclosed by epithelial tissues arises from an interplay of lumen pressure, mechanics of the cell cortex, and cell-cell adhesion. Here, we explore the mechanical basis for the control of this interplay using the shape space of MDCK cysts and the instability of their apical surfaces under tight junction perturbations [Mukenhirn et al., Dev. Cell 59, 2886 (2024)]. We discover that the cysts respond to these perturbations by significantly modulating their lateral and basal tensions, in addition to the known modulations of pressure and apical belt tension. We develop a mean-field three-dimensional vertex model of these cysts that reproduces the experimental shape instability quantitatively. This reveals that the observed increase of lateral contractility is a cellular response that counters the instability. Our work thus shows how regulation of the mechanics of all cell surfaces conspires to control lumen morphology. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2509_04316 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Control of lumen morphology by lateral and basal cell surfaces Ray, Chandraniva Guha Mukenhirn, Markus Honigmann, Alf Haas, Pierre A. Soft Condensed Matter Biological Physics Tissues and Organs Across development, the morphology of fluid-filled lumina enclosed by epithelial tissues arises from an interplay of lumen pressure, mechanics of the cell cortex, and cell-cell adhesion. Here, we explore the mechanical basis for the control of this interplay using the shape space of MDCK cysts and the instability of their apical surfaces under tight junction perturbations [Mukenhirn et al., Dev. Cell 59, 2886 (2024)]. We discover that the cysts respond to these perturbations by significantly modulating their lateral and basal tensions, in addition to the known modulations of pressure and apical belt tension. We develop a mean-field three-dimensional vertex model of these cysts that reproduces the experimental shape instability quantitatively. This reveals that the observed increase of lateral contractility is a cellular response that counters the instability. Our work thus shows how regulation of the mechanics of all cell surfaces conspires to control lumen morphology. |
| title | Control of lumen morphology by lateral and basal cell surfaces |
| topic | Soft Condensed Matter Biological Physics Tissues and Organs |
| url | https://arxiv.org/abs/2509.04316 |