Continuous scanning full-field OCT for fast volumetric imaging of multi-cellular aggregates
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arXiv
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| Main Authors: | , , |
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| Format: | Preprint |
| Published: |
2025
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| _version_ | 1866908528600416256 |
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| author | Kersuzan, Charlie Jana, Anirban Badon, Amaury |
| author_facet | Kersuzan, Charlie Jana, Anirban Badon, Amaury |
| contents | Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method that eliminates piezoelectric displacement and synchronization by continuously translating the sample with a motorized stage while recording images on the fly. Depth-resolved information is retrieved via Fourier analysis of the temporal signal at each pixel. This approach enables volumetric imaging over several hundred micrometers within tens of seconds and provides a higher contrast-to-noise ratio than traditional four-phase FF-OCT. Continuous-scanning FF-OCT thus represents a simpler and faster alternative for 3D bio-imaging of living tissues and organoids. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2509_08101 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Continuous scanning full-field OCT for fast volumetric imaging of multi-cellular aggregates Kersuzan, Charlie Jana, Anirban Badon, Amaury Optics Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method that eliminates piezoelectric displacement and synchronization by continuously translating the sample with a motorized stage while recording images on the fly. Depth-resolved information is retrieved via Fourier analysis of the temporal signal at each pixel. This approach enables volumetric imaging over several hundred micrometers within tens of seconds and provides a higher contrast-to-noise ratio than traditional four-phase FF-OCT. Continuous-scanning FF-OCT thus represents a simpler and faster alternative for 3D bio-imaging of living tissues and organoids. |
| title | Continuous scanning full-field OCT for fast volumetric imaging of multi-cellular aggregates |
| topic | Optics |
| url | https://arxiv.org/abs/2509.08101 |