Quantifying the liquid-liquid transition in cold water/glycerol mixtures by ih-RIDME
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arXiv
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| Format: | Preprint |
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2025
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| _version_ | 1866912580627333120 |
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| author | Kuzin, Sergei Yulikov, Maxim |
| author_facet | Kuzin, Sergei Yulikov, Maxim |
| contents | Water/glycerol mixtures are common for experiments with biomacromolecules at cryogenic temperatures due to their vitrification properties. Above the glass transition temperature, they undergo liquid-liquid phase separation. Using the novel EPR technique called intermolecular hyperfine Relaxation-Induced Dipolar Modulation Enhancement (ih-RIDME), we quantified the molar composition in frozen water/glycerol mixtures with one or the other component deuterated after the phase transition. Our experiments reveal nearly equal phase composition regardless of the proton/deuterium isotope balance. With the new ih-RIDME data, we can also revisit the already reported body of glass transition data for such mixtures and build a consistent picture for water/glycerol freezing and phase transitions. Our results also indicate that ih-RIDME has the potential for investigating the solvation shells of spin-labelled macromolecules. |
| format | Preprint |
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arxiv_https___arxiv_org_abs_2509_08633 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Quantifying the liquid-liquid transition in cold water/glycerol mixtures by ih-RIDME Kuzin, Sergei Yulikov, Maxim Soft Condensed Matter Chemical Physics Biomolecules Water/glycerol mixtures are common for experiments with biomacromolecules at cryogenic temperatures due to their vitrification properties. Above the glass transition temperature, they undergo liquid-liquid phase separation. Using the novel EPR technique called intermolecular hyperfine Relaxation-Induced Dipolar Modulation Enhancement (ih-RIDME), we quantified the molar composition in frozen water/glycerol mixtures with one or the other component deuterated after the phase transition. Our experiments reveal nearly equal phase composition regardless of the proton/deuterium isotope balance. With the new ih-RIDME data, we can also revisit the already reported body of glass transition data for such mixtures and build a consistent picture for water/glycerol freezing and phase transitions. Our results also indicate that ih-RIDME has the potential for investigating the solvation shells of spin-labelled macromolecules. |
| title | Quantifying the liquid-liquid transition in cold water/glycerol mixtures by ih-RIDME |
| topic | Soft Condensed Matter Chemical Physics Biomolecules |
| url | https://arxiv.org/abs/2509.08633 |