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| Autori principali: | , , , , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2025
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| Soggetti: | |
| Accesso online: | https://arxiv.org/abs/2510.00267 |
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| _version_ | 1866908571198816256 |
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| author | Fay, Victoria Pu, Ye Tzang, Omer Caravaca, Antonio Piestun, Rafael Tolstonog, Genrich Simon, Christian Psaltis, Demetri Moser, Christophe |
| author_facet | Fay, Victoria Pu, Ye Tzang, Omer Caravaca, Antonio Piestun, Rafael Tolstonog, Genrich Simon, Christian Psaltis, Demetri Moser, Christophe |
| contents | Endoscopic optical imaging using a single multimode fiber (MMF) has emerged as a promising approach for highly compact, minimally invasive, and high-resolution imaging. Unlike conventional fiber bundles, MMF-based endomicroscopes exploit the controlled excitation of multiple spatially overlapping modes in a single MMF. of core diameters of tens of micrometers. to deliver and collect light to form images with sub-micrometer resolution. Here, we introduce a fluorescence lifetime imaging microscopy (FLIM) modality to the MMF endomicroscope. We use amplitude modulation of a 405 nm single-mode light source at radio frequency (RF) and lock-in detection of autofluorescence to obtain intensity and lifetime images at sub-micrometer resolution. We experimentally demonstrate the capability of the ultrathin endomicroscope to perform label-free imaging in thick ex vivo murine submandibular gland tissue. With a temporal resolution of 0.03 ns, the FLIM images show distinguished structures of lifetime differences down to 0.5 ns. The combination of sub-micrometer fluorescence intensity and lifetime images in a minimally invasive endomicroscope opens new avenues for label-free cancer detection. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2510_00267 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | High resolution Fluorescence lifetime IMaging Micro-Endoscopy (FLIMME) using a single multimode fiber Fay, Victoria Pu, Ye Tzang, Omer Caravaca, Antonio Piestun, Rafael Tolstonog, Genrich Simon, Christian Psaltis, Demetri Moser, Christophe Optics Endoscopic optical imaging using a single multimode fiber (MMF) has emerged as a promising approach for highly compact, minimally invasive, and high-resolution imaging. Unlike conventional fiber bundles, MMF-based endomicroscopes exploit the controlled excitation of multiple spatially overlapping modes in a single MMF. of core diameters of tens of micrometers. to deliver and collect light to form images with sub-micrometer resolution. Here, we introduce a fluorescence lifetime imaging microscopy (FLIM) modality to the MMF endomicroscope. We use amplitude modulation of a 405 nm single-mode light source at radio frequency (RF) and lock-in detection of autofluorescence to obtain intensity and lifetime images at sub-micrometer resolution. We experimentally demonstrate the capability of the ultrathin endomicroscope to perform label-free imaging in thick ex vivo murine submandibular gland tissue. With a temporal resolution of 0.03 ns, the FLIM images show distinguished structures of lifetime differences down to 0.5 ns. The combination of sub-micrometer fluorescence intensity and lifetime images in a minimally invasive endomicroscope opens new avenues for label-free cancer detection. |
| title | High resolution Fluorescence lifetime IMaging Micro-Endoscopy (FLIMME) using a single multimode fiber |
| topic | Optics |
| url | https://arxiv.org/abs/2510.00267 |