Full-Field Brillouin Microscopy with a Scanning Fabry-Perot Interferometer
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arXiv
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| Format: | Preprint |
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2025
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| _version_ | 1866910151010680832 |
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| author | Pochylski, Mikolaj |
| author_facet | Pochylski, Mikolaj |
| contents | Brillouin microscopy is an emerging optical technique for probing mechanical properties with submicron resolution, offering fully non-contact, label-free operation. Despite its unique capabilities, broader adoption has been limited by slow acquisition speeds, particularly in systems based on scanning Fabry-Perot interferometers (FPIs). Based on prior implementations, FPIs have typically been considered too slow for practical imaging, particularly when both spatial and spectral precision are required. Here, we demonstrate that a standard multi-pass tandem FPI can be repurposed for full-field Brillouin imaging when operated in a spectral filtering mode. Combined with light-sheet illumination for uniform, low-dose excitation, this configuration enables rapid, spatially resolved acquisition of Brillouin spectra. By restricting scanning to a narrow frequency range around the Brillouin peak, we acquired a full 2D image within one minute, achieving millisecond-scale single pixel dwell times and micrometer-scale spatial resolution. The system uniquely supports Brillouin emission imaging at selected frequency shifts, a capability not available with other spontaneous Brillouin implementations. Results from synthetic and biological specimens demonstrate how existing FPI-based setups can be extended to full-field imaging and outline a pathway toward future dedicated FPI instruments optimized for high-speed, high-contrast Brillouin microscopy. |
| format | Preprint |
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arxiv_https___arxiv_org_abs_2510_00700 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Full-Field Brillouin Microscopy with a Scanning Fabry-Perot Interferometer Pochylski, Mikolaj Optics Instrumentation and Detectors Brillouin microscopy is an emerging optical technique for probing mechanical properties with submicron resolution, offering fully non-contact, label-free operation. Despite its unique capabilities, broader adoption has been limited by slow acquisition speeds, particularly in systems based on scanning Fabry-Perot interferometers (FPIs). Based on prior implementations, FPIs have typically been considered too slow for practical imaging, particularly when both spatial and spectral precision are required. Here, we demonstrate that a standard multi-pass tandem FPI can be repurposed for full-field Brillouin imaging when operated in a spectral filtering mode. Combined with light-sheet illumination for uniform, low-dose excitation, this configuration enables rapid, spatially resolved acquisition of Brillouin spectra. By restricting scanning to a narrow frequency range around the Brillouin peak, we acquired a full 2D image within one minute, achieving millisecond-scale single pixel dwell times and micrometer-scale spatial resolution. The system uniquely supports Brillouin emission imaging at selected frequency shifts, a capability not available with other spontaneous Brillouin implementations. Results from synthetic and biological specimens demonstrate how existing FPI-based setups can be extended to full-field imaging and outline a pathway toward future dedicated FPI instruments optimized for high-speed, high-contrast Brillouin microscopy. |
| title | Full-Field Brillouin Microscopy with a Scanning Fabry-Perot Interferometer |
| topic | Optics Instrumentation and Detectors |
| url | https://arxiv.org/abs/2510.00700 |