Molecularly imprinted nanopores for multiplexed sensing, release, and in-edge computing

Fuente: arXiv
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Main Authors: Douaki, Ali, Weng, Shukun, Dante, Silvia, Nakatsuka, Nako, Tsutsui, Makusu, Krahne, Roman, Garoli, Denis
Format: Preprint
Published: 2025
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author Douaki, Ali
Weng, Shukun
Dante, Silvia
Nakatsuka, Nako
Tsutsui, Makusu
Krahne, Roman
Garoli, Denis
author_facet Douaki, Ali
Weng, Shukun
Dante, Silvia
Nakatsuka, Nako
Tsutsui, Makusu
Krahne, Roman
Garoli, Denis
contents In nanopore technology, the development of multiplexed detection and release platforms with high spatial and temporal resolution remains a significant challenge due to the difficulty in distinguishing signals originating from different nanopores in a single chip. In this work, we present a solid-state nanopore system functionalized with molecularly imprinted polymers (MIPs) for the selective detection and controlled release of neurotransmitters. We designed a nanopore array where each nanopore is functionalized with a specific MIP able to recognize specific neurotransmitters (dopamine, gamma-aminobutyric acid, and histamine, respectively). The platform demonstrated high performance in terms of sensitivity, selectivity, recovery, and stability. Multiplexed detection with high spatiotemporal resolution of the order of 100 ms/ 3 μm was achieved by specifically depositing MIPs and conductive hydrogels on different nanopores prepared on a single solid-state membrane. The employment of micro-chambers for each nanopore prevented signal cross-talk, thereby enabling simultaneous detection and release of multiple neurotransmitters. Moreover, we demonstrated computing with different logic gates and in-edge computing. This nanopore platform represents a radically novel approach towards hybrid solid-state nanopores able to perform real-time label-free multiplex detection, controlled biomolecule release, and ionic logic computing, addressing key challenges in neurochemical sensing and bio-computation.
format Preprint
id arxiv_https___arxiv_org_abs_2510_13490
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Molecularly imprinted nanopores for multiplexed sensing, release, and in-edge computing
Douaki, Ali
Weng, Shukun
Dante, Silvia
Nakatsuka, Nako
Tsutsui, Makusu
Krahne, Roman
Garoli, Denis
Applied Physics
In nanopore technology, the development of multiplexed detection and release platforms with high spatial and temporal resolution remains a significant challenge due to the difficulty in distinguishing signals originating from different nanopores in a single chip. In this work, we present a solid-state nanopore system functionalized with molecularly imprinted polymers (MIPs) for the selective detection and controlled release of neurotransmitters. We designed a nanopore array where each nanopore is functionalized with a specific MIP able to recognize specific neurotransmitters (dopamine, gamma-aminobutyric acid, and histamine, respectively). The platform demonstrated high performance in terms of sensitivity, selectivity, recovery, and stability. Multiplexed detection with high spatiotemporal resolution of the order of 100 ms/ 3 μm was achieved by specifically depositing MIPs and conductive hydrogels on different nanopores prepared on a single solid-state membrane. The employment of micro-chambers for each nanopore prevented signal cross-talk, thereby enabling simultaneous detection and release of multiple neurotransmitters. Moreover, we demonstrated computing with different logic gates and in-edge computing. This nanopore platform represents a radically novel approach towards hybrid solid-state nanopores able to perform real-time label-free multiplex detection, controlled biomolecule release, and ionic logic computing, addressing key challenges in neurochemical sensing and bio-computation.
title Molecularly imprinted nanopores for multiplexed sensing, release, and in-edge computing
topic Applied Physics
url https://arxiv.org/abs/2510.13490