Constrained Diffusion for Protein Design with Hard Structural Constraints

Fuente: arXiv
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Main Authors: Christopher, Jacob K., Seamann, Austin, Cui, Jingyi, Khare, Sagar, Fioretto, Ferdinando
Format: Preprint
Published: 2025
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author Christopher, Jacob K.
Seamann, Austin
Cui, Jingyi
Khare, Sagar
Fioretto, Ferdinando
author_facet Christopher, Jacob K.
Seamann, Austin
Cui, Jingyi
Khare, Sagar
Fioretto, Ferdinando
contents Diffusion models offer a powerful means of capturing the manifold of realistic protein structures, enabling rapid design for protein engineering tasks. However, existing approaches observe critical failure modes when precise constraints are necessary for functional design. To this end, we present a constrained diffusion framework for structure-guided protein design, ensuring strict adherence to functional requirements while maintaining precise stereochemical and geometric feasibility. The approach integrates proximal feasibility updates with ADMM decomposition into the generative process, scaling effectively to the complex constraint sets of this domain. We evaluate on challenging protein design tasks, including motif scaffolding and vacancy-constrained pocket design, while introducing a novel curated benchmark dataset for motif scaffolding in the PDZ domain. Our approach achieves state-of-the-art, providing perfect satisfaction of bonding and geometric constraints with no degradation in structural diversity.
format Preprint
id arxiv_https___arxiv_org_abs_2510_14989
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Constrained Diffusion for Protein Design with Hard Structural Constraints
Christopher, Jacob K.
Seamann, Austin
Cui, Jingyi
Khare, Sagar
Fioretto, Ferdinando
Biomolecules
Artificial Intelligence
Machine Learning
Diffusion models offer a powerful means of capturing the manifold of realistic protein structures, enabling rapid design for protein engineering tasks. However, existing approaches observe critical failure modes when precise constraints are necessary for functional design. To this end, we present a constrained diffusion framework for structure-guided protein design, ensuring strict adherence to functional requirements while maintaining precise stereochemical and geometric feasibility. The approach integrates proximal feasibility updates with ADMM decomposition into the generative process, scaling effectively to the complex constraint sets of this domain. We evaluate on challenging protein design tasks, including motif scaffolding and vacancy-constrained pocket design, while introducing a novel curated benchmark dataset for motif scaffolding in the PDZ domain. Our approach achieves state-of-the-art, providing perfect satisfaction of bonding and geometric constraints with no degradation in structural diversity.
title Constrained Diffusion for Protein Design with Hard Structural Constraints
topic Biomolecules
Artificial Intelligence
Machine Learning
url https://arxiv.org/abs/2510.14989