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| Autores principales: | , , , , , , |
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| Formato: | Preprint |
| Publicado: |
2025
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| Materias: | |
| Acceso en línea: | https://arxiv.org/abs/2510.15579 |
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| _version_ | 1866909852712828928 |
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| author | Soltaninezhad, Mohammad Rouzbahani, Yashar Contreras, Jhonatan Chippalkatti, Rohan Abankwa, Daniel Kwaku Eggeling, Christian Bocklitz, Thomas |
| author_facet | Soltaninezhad, Mohammad Rouzbahani, Yashar Contreras, Jhonatan Chippalkatti, Rohan Abankwa, Daniel Kwaku Eggeling, Christian Bocklitz, Thomas |
| contents | Lightweight deep learning models offer substantial reductions in computational cost and environmental impact, making them crucial for scientific applications. We present a lightweight CycleGAN for modality transfer in fluorescence microscopy (confocal to super-resolution STED/deconvolved STED), addressing the common challenge of unpaired datasets. By replacing the traditional channel-doubling strategy in the U-Net-based generator with a fixed channel approach, we drastically reduce trainable parameters from 41.8 million to approximately nine thousand, achieving superior performance with faster training and lower memory usage. We also introduce the GAN as a diagnostic tool for experimental and labeling quality. When trained on high-quality images, the GAN learns the characteristics of optimal imaging; deviations between its generated outputs and new experimental images can reveal issues such as photobleaching, artifacts, or inaccurate labeling. This establishes the model as a practical tool for validating experimental accuracy and image fidelity in microscopy workflows. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2510_15579 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Lightweight CycleGAN Models for Cross-Modality Image Transformation and Experimental Quality Assessment in Fluorescence Microscopy Soltaninezhad, Mohammad Rouzbahani, Yashar Contreras, Jhonatan Chippalkatti, Rohan Abankwa, Daniel Kwaku Eggeling, Christian Bocklitz, Thomas Computer Vision and Pattern Recognition Artificial Intelligence Lightweight deep learning models offer substantial reductions in computational cost and environmental impact, making them crucial for scientific applications. We present a lightweight CycleGAN for modality transfer in fluorescence microscopy (confocal to super-resolution STED/deconvolved STED), addressing the common challenge of unpaired datasets. By replacing the traditional channel-doubling strategy in the U-Net-based generator with a fixed channel approach, we drastically reduce trainable parameters from 41.8 million to approximately nine thousand, achieving superior performance with faster training and lower memory usage. We also introduce the GAN as a diagnostic tool for experimental and labeling quality. When trained on high-quality images, the GAN learns the characteristics of optimal imaging; deviations between its generated outputs and new experimental images can reveal issues such as photobleaching, artifacts, or inaccurate labeling. This establishes the model as a practical tool for validating experimental accuracy and image fidelity in microscopy workflows. |
| title | Lightweight CycleGAN Models for Cross-Modality Image Transformation and Experimental Quality Assessment in Fluorescence Microscopy |
| topic | Computer Vision and Pattern Recognition Artificial Intelligence |
| url | https://arxiv.org/abs/2510.15579 |