Interactive visualization of kidney micro-compartmental segmentations and associated pathomics on whole slide images

Fuente: arXiv
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Autori principali: Keller, Mark S., Lucarelli, Nicholas, Chen, Yijiang, Border, Samuel, Janowczyk, Andrew, Himmelfarb, Jonathan, Kretzler, Matthias, Hodgin, Jeffrey, Barisoni, Laura, Demeke, Dawit, Herlitz, Leal, Moeckel, Gilbert, Rosenberg, Avi Z., Ding, Yanli, Sarder, Pinaki, Gehlenborg, Nils
Natura: Preprint
Pubblicazione: 2025
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author Keller, Mark S.
Lucarelli, Nicholas
Chen, Yijiang
Border, Samuel
Janowczyk, Andrew
Himmelfarb, Jonathan
Kretzler, Matthias
Hodgin, Jeffrey
Barisoni, Laura
Demeke, Dawit
Herlitz, Leal
Moeckel, Gilbert
Rosenberg, Avi Z.
Ding, Yanli
Sarder, Pinaki
Gehlenborg, Nils
author_facet Keller, Mark S.
Lucarelli, Nicholas
Chen, Yijiang
Border, Samuel
Janowczyk, Andrew
Himmelfarb, Jonathan
Kretzler, Matthias
Hodgin, Jeffrey
Barisoni, Laura
Demeke, Dawit
Herlitz, Leal
Moeckel, Gilbert
Rosenberg, Avi Z.
Ding, Yanli
Sarder, Pinaki
Gehlenborg, Nils
contents Application of machine learning techniques enables segmentation of functional tissue units in histology whole-slide images (WSIs). We built a pipeline to apply previously validated segmentation models of kidney structures and extract quantitative features from these structures. Such quantitative analysis also requires qualitative inspection of results for quality control, exploration, and communication. We extend the Vitessce web-based visualization tool to enable visualization of segmentations of multiple types of functional tissue units, such as, glomeruli, tubules, arteries/arterioles in the kidney. Moreover, we propose a standard representation for files containing multiple segmentation bitmasks, which we define polymorphically, such that existing formats including OME-TIFF, OME-NGFF, AnnData, MuData, and SpatialData can be used. We demonstrate that these methods enable researchers and the broader public to interactively explore datasets containing multiple segmented entities and associated features, including for exploration of renal morphometry of biopsies from the Kidney Precision Medicine Project (KPMP) and the Human Biomolecular Atlas Program (HuBMAP).
format Preprint
id arxiv_https___arxiv_org_abs_2510_19499
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Interactive visualization of kidney micro-compartmental segmentations and associated pathomics on whole slide images
Keller, Mark S.
Lucarelli, Nicholas
Chen, Yijiang
Border, Samuel
Janowczyk, Andrew
Himmelfarb, Jonathan
Kretzler, Matthias
Hodgin, Jeffrey
Barisoni, Laura
Demeke, Dawit
Herlitz, Leal
Moeckel, Gilbert
Rosenberg, Avi Z.
Ding, Yanli
Sarder, Pinaki
Gehlenborg, Nils
Quantitative Methods
Human-Computer Interaction
Application of machine learning techniques enables segmentation of functional tissue units in histology whole-slide images (WSIs). We built a pipeline to apply previously validated segmentation models of kidney structures and extract quantitative features from these structures. Such quantitative analysis also requires qualitative inspection of results for quality control, exploration, and communication. We extend the Vitessce web-based visualization tool to enable visualization of segmentations of multiple types of functional tissue units, such as, glomeruli, tubules, arteries/arterioles in the kidney. Moreover, we propose a standard representation for files containing multiple segmentation bitmasks, which we define polymorphically, such that existing formats including OME-TIFF, OME-NGFF, AnnData, MuData, and SpatialData can be used. We demonstrate that these methods enable researchers and the broader public to interactively explore datasets containing multiple segmented entities and associated features, including for exploration of renal morphometry of biopsies from the Kidney Precision Medicine Project (KPMP) and the Human Biomolecular Atlas Program (HuBMAP).
title Interactive visualization of kidney micro-compartmental segmentations and associated pathomics on whole slide images
topic Quantitative Methods
Human-Computer Interaction
url https://arxiv.org/abs/2510.19499