Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology
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arXiv
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| Autori principali: | , , , , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2025
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| author | Lee, Minseok Lifshitz, Tal Lee, Young Ki Kim, Geon Park, Seog Yun Lee, Hayoung Park, Juyeon Lee, Eun Kyung Park, YongKeun |
| author_facet | Lee, Minseok Lifshitz, Tal Lee, Young Ki Kim, Geon Park, Seog Yun Lee, Hayoung Park, Juyeon Lee, Eun Kyung Park, YongKeun |
| contents | Conventional bright-field (BF) cytology of thyroid fine-needle aspiration biopsy (FNAB) suffers from staining variability and limited subcellular contrast. Here, we present a refractive index-correlated pseudocoloring (RICP) framework that integrates quantitative refractive index (RI) maps obtained by holotomography (HT) with color BF images to enhance diagnostic interpretability. The imaging platform combines a digital micromirror device (DMD)-based HT system with an RGB LED illumination module, enabling simultaneous acquisition of RI tomograms and BF images from PAP-stained thyroid samples. The RICP algorithm adaptively embeds RI-derived structural information into the least-occupied hue channel, preserving color fidelity while enhancing nuclear and cytoplasmic contrast. Applied to benign and malignant thyroid clusters, RICP revealed diagnostically relevant features such as nucleoli, lipid droplets, and nuclear irregularities, and hue-saturation analysis quantitatively differentiated cytological categories. This perceptually grounded, label-free framework bridges conventional color cytology and quantitative optical imaging for improved diagnostic precision. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2510_26356 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology Lee, Minseok Lifshitz, Tal Lee, Young Ki Kim, Geon Park, Seog Yun Lee, Hayoung Park, Juyeon Lee, Eun Kyung Park, YongKeun Optics Conventional bright-field (BF) cytology of thyroid fine-needle aspiration biopsy (FNAB) suffers from staining variability and limited subcellular contrast. Here, we present a refractive index-correlated pseudocoloring (RICP) framework that integrates quantitative refractive index (RI) maps obtained by holotomography (HT) with color BF images to enhance diagnostic interpretability. The imaging platform combines a digital micromirror device (DMD)-based HT system with an RGB LED illumination module, enabling simultaneous acquisition of RI tomograms and BF images from PAP-stained thyroid samples. The RICP algorithm adaptively embeds RI-derived structural information into the least-occupied hue channel, preserving color fidelity while enhancing nuclear and cytoplasmic contrast. Applied to benign and malignant thyroid clusters, RICP revealed diagnostically relevant features such as nucleoli, lipid droplets, and nuclear irregularities, and hue-saturation analysis quantitatively differentiated cytological categories. This perceptually grounded, label-free framework bridges conventional color cytology and quantitative optical imaging for improved diagnostic precision. |
| title | Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology |
| topic | Optics |
| url | https://arxiv.org/abs/2510.26356 |