Molecular Dynamics Simulations of Membrane Selectivity of Star Peptides Across Different Bacterial and Mammalian Bilipids

Fuente: arXiv
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Main Authors: Jayawardena, Amal, Hung, Andrew, Qiao, Greg, OBrien-Simpson, Neil, Hajizadeh, Elnaz
Format: Preprint
Published: 2025
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_version_ 1866914143299174400
author Jayawardena, Amal
Hung, Andrew
Qiao, Greg
OBrien-Simpson, Neil
Hajizadeh, Elnaz
author_facet Jayawardena, Amal
Hung, Andrew
Qiao, Greg
OBrien-Simpson, Neil
Hajizadeh, Elnaz
contents Structurally nanoengineered antimicrobial peptide polymers (SNAPPs) are emerging as promising selective agents against bacterial membranes. In this study, we used all atom molecular dynamics simulation techniques to investigate the interaction of a promising cationic SNAPP architecture (Alt-SNAPP with 8 arms made of alternating lysine and valine residues) with modelled Gram-negative, Gram-positive, mammalian, and red blood cell membranes. Alt-SNAPP exhibited rapid and stable binding to bacterial membranes, driven by electrostatic interactions with anionic lipids such as phosphatidylglycerol (PG) and cardiolipin (CL), and supported by membrane fluidity. In contrast, mammalian and red blood cell membranes, enriched in zwitterionic lipids and cholesterol, resisted peptide association entirely. Analyses of center of mass distance, partial density, hydrogen bonding, and interaction energy confirmed that SNAPP remains fully excluded from host like membranes while forming stable, multivalent interactions with bacterial bilayers. These findings provide mechanistic insight into membrane selectivity of SNAPP and offer a molecular framework for designing next generation antimicrobial polymers with minimal off target toxicity.
format Preprint
id arxiv_https___arxiv_org_abs_2511_05513
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Molecular Dynamics Simulations of Membrane Selectivity of Star Peptides Across Different Bacterial and Mammalian Bilipids
Jayawardena, Amal
Hung, Andrew
Qiao, Greg
OBrien-Simpson, Neil
Hajizadeh, Elnaz
Biomolecules
Materials Science
Soft Condensed Matter
Structurally nanoengineered antimicrobial peptide polymers (SNAPPs) are emerging as promising selective agents against bacterial membranes. In this study, we used all atom molecular dynamics simulation techniques to investigate the interaction of a promising cationic SNAPP architecture (Alt-SNAPP with 8 arms made of alternating lysine and valine residues) with modelled Gram-negative, Gram-positive, mammalian, and red blood cell membranes. Alt-SNAPP exhibited rapid and stable binding to bacterial membranes, driven by electrostatic interactions with anionic lipids such as phosphatidylglycerol (PG) and cardiolipin (CL), and supported by membrane fluidity. In contrast, mammalian and red blood cell membranes, enriched in zwitterionic lipids and cholesterol, resisted peptide association entirely. Analyses of center of mass distance, partial density, hydrogen bonding, and interaction energy confirmed that SNAPP remains fully excluded from host like membranes while forming stable, multivalent interactions with bacterial bilayers. These findings provide mechanistic insight into membrane selectivity of SNAPP and offer a molecular framework for designing next generation antimicrobial polymers with minimal off target toxicity.
title Molecular Dynamics Simulations of Membrane Selectivity of Star Peptides Across Different Bacterial and Mammalian Bilipids
topic Biomolecules
Materials Science
Soft Condensed Matter
url https://arxiv.org/abs/2511.05513