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Autores principales: Afrough, Armin, Andersen, Pernille, Weihrauch, Tanja Ninette Angelika, Juhl, Dennis Wilkens, Bakalis, Serafim, Malmos, Kirsten Gade, Vosegaard, Thomas
Formato: Preprint
Publicado: 2025
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Acceso en línea:https://arxiv.org/abs/2511.06834
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author Afrough, Armin
Andersen, Pernille
Weihrauch, Tanja Ninette Angelika
Juhl, Dennis Wilkens
Bakalis, Serafim
Malmos, Kirsten Gade
Vosegaard, Thomas
author_facet Afrough, Armin
Andersen, Pernille
Weihrauch, Tanja Ninette Angelika
Juhl, Dennis Wilkens
Bakalis, Serafim
Malmos, Kirsten Gade
Vosegaard, Thomas
contents Lactose is the major component of milk powders and is normally found to be in a glassy/amorphous state. During storage, lactose is known to participate in physicochemical processes, including crystallization on the surface and reaction with proteins such as $β$-lactoglobulin. Lactose needs to be mobile to participate in such processes. However, there is a lack of evidence of its mobility in milk powders. In this study, we demonstrate that some of the lactose becomes mobile when milk powders are exposed to humid air $-$ an inappropriate storage condition. This mobility is evidenced by peaks in magic angle spinning $^{1}\mathrm{H}$ NMR spectra of milk powders in the range of 3.5 ppm to 4.0 ppm, which stem from lactose molecules displaying considerable rotational mobility. These signals have a longitudinal relaxation time constant T1 similar to that of mobile water according to 2D T1$-δ(^{1}\mathrm{H})$ experiments under magic angle spinning. Furthermore, 2D $^{1}\mathrm{H}-^{13}\mathrm{C}$ HSQC magic angle spinning experiments of skim milk powder demonstrate the same fingerprint as that of lactose in the solution, confirming our observations.
format Preprint
id arxiv_https___arxiv_org_abs_2511_06834
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Mobility of Lactose in Milk Powders
Afrough, Armin
Andersen, Pernille
Weihrauch, Tanja Ninette Angelika
Juhl, Dennis Wilkens
Bakalis, Serafim
Malmos, Kirsten Gade
Vosegaard, Thomas
Soft Condensed Matter
Lactose is the major component of milk powders and is normally found to be in a glassy/amorphous state. During storage, lactose is known to participate in physicochemical processes, including crystallization on the surface and reaction with proteins such as $β$-lactoglobulin. Lactose needs to be mobile to participate in such processes. However, there is a lack of evidence of its mobility in milk powders. In this study, we demonstrate that some of the lactose becomes mobile when milk powders are exposed to humid air $-$ an inappropriate storage condition. This mobility is evidenced by peaks in magic angle spinning $^{1}\mathrm{H}$ NMR spectra of milk powders in the range of 3.5 ppm to 4.0 ppm, which stem from lactose molecules displaying considerable rotational mobility. These signals have a longitudinal relaxation time constant T1 similar to that of mobile water according to 2D T1$-δ(^{1}\mathrm{H})$ experiments under magic angle spinning. Furthermore, 2D $^{1}\mathrm{H}-^{13}\mathrm{C}$ HSQC magic angle spinning experiments of skim milk powder demonstrate the same fingerprint as that of lactose in the solution, confirming our observations.
title Mobility of Lactose in Milk Powders
topic Soft Condensed Matter
url https://arxiv.org/abs/2511.06834