Seek and You Shall Fold

Fuente: arXiv
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Autori principali: Sellam, Nadav Bojan, Bojan, Meital, Schanda, Paul, Bronstein, Alex
Natura: Preprint
Pubblicazione: 2025
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author Sellam, Nadav Bojan
Bojan, Meital
Schanda, Paul
Bronstein, Alex
author_facet Sellam, Nadav Bojan
Bojan, Meital
Schanda, Paul
Bronstein, Alex
contents Accurate protein structures are essential for understanding biological function, yet incorporating experimental data into protein generative models remains a major challenge. Most predictors of experimental observables are non-differentiable, making them incompatible with gradient-based conditional sampling. This is especially limiting in nuclear magnetic resonance, where rich data such as chemical shifts are hard to directly integrate into generative modeling. We introduce a framework for non-differentiable guidance of protein generative models, coupling a continuous diffusion-based generator with any black-box objective via a tailored genetic algorithm. We demonstrate its effectiveness across three modalities: pairwise distance constraints, nuclear Overhauser effect restraints, and for the first time chemical shifts. These results establish chemical shift guided structure generation as feasible, expose key weaknesses in current predictors, and showcase a general strategy for incorporating diverse experimental signals. Our work points toward automated, data-conditioned protein modeling beyond the limits of differentiability.
format Preprint
id arxiv_https___arxiv_org_abs_2511_13244
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Seek and You Shall Fold
Sellam, Nadav Bojan
Bojan, Meital
Schanda, Paul
Bronstein, Alex
Machine Learning
Artificial Intelligence
Accurate protein structures are essential for understanding biological function, yet incorporating experimental data into protein generative models remains a major challenge. Most predictors of experimental observables are non-differentiable, making them incompatible with gradient-based conditional sampling. This is especially limiting in nuclear magnetic resonance, where rich data such as chemical shifts are hard to directly integrate into generative modeling. We introduce a framework for non-differentiable guidance of protein generative models, coupling a continuous diffusion-based generator with any black-box objective via a tailored genetic algorithm. We demonstrate its effectiveness across three modalities: pairwise distance constraints, nuclear Overhauser effect restraints, and for the first time chemical shifts. These results establish chemical shift guided structure generation as feasible, expose key weaknesses in current predictors, and showcase a general strategy for incorporating diverse experimental signals. Our work points toward automated, data-conditioned protein modeling beyond the limits of differentiability.
title Seek and You Shall Fold
topic Machine Learning
Artificial Intelligence
url https://arxiv.org/abs/2511.13244