A Versatile Optical Frontend for Multicolor Fluorescence Imaging with Miniaturized Lensless Sensors
Fuente:
arXiv
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | Preprint |
| Publicado: |
2025
|
| Materias: | |
| Acceso en línea: | |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1866910027084726272 |
|---|---|
| author | Harris, Lukas Roschelle, Micah Bartley, Jack Anwar, Mekhail |
| author_facet | Harris, Lukas Roschelle, Micah Bartley, Jack Anwar, Mekhail |
| contents | Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing fluorescent emissions. However, conventional thin-film interference filters are sensitive to angle of incidence (AOI), complicating their use in lensless systems. Here we thoroughly analyze and optimize a technique using a fiber optic plate (FOP) to absorb off-axis light that would bleed through the interference filter while improving image resolution. Through simulations, we show that the numerical aperture (NA) of the FOP drives inherent design tradeoffs: collection efficiency improves rapidly with a higher NA, but at the cost of resolution, increased device thickness, and fluorescence excitation efficiency. To illustrate this, we optimize two optical frontends with full-width at half maximums (FWHMs) of 8.3° and 45.7°. Implementing these designs, we show that angle-insensitivity requires filters on both sides of the FOP, due to scattering. In imaging experiments, the 520-$μ$m-thick high-NA design is 59$\times$ more sensitive to fluorescence while only degrading resolution by 3.2$\times$. Alternatively, the low-NA design is capable of three-color fluorescence imaging with 110-$μ$m resolution at a 1-mm working distance. Overall, we demonstrate a versatile optical frontend that is adaptable to a range of applications using different fluorophores, illumination configurations, and lensless imaging techniques. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2511_17860 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | A Versatile Optical Frontend for Multicolor Fluorescence Imaging with Miniaturized Lensless Sensors Harris, Lukas Roschelle, Micah Bartley, Jack Anwar, Mekhail Image and Video Processing Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing fluorescent emissions. However, conventional thin-film interference filters are sensitive to angle of incidence (AOI), complicating their use in lensless systems. Here we thoroughly analyze and optimize a technique using a fiber optic plate (FOP) to absorb off-axis light that would bleed through the interference filter while improving image resolution. Through simulations, we show that the numerical aperture (NA) of the FOP drives inherent design tradeoffs: collection efficiency improves rapidly with a higher NA, but at the cost of resolution, increased device thickness, and fluorescence excitation efficiency. To illustrate this, we optimize two optical frontends with full-width at half maximums (FWHMs) of 8.3° and 45.7°. Implementing these designs, we show that angle-insensitivity requires filters on both sides of the FOP, due to scattering. In imaging experiments, the 520-$μ$m-thick high-NA design is 59$\times$ more sensitive to fluorescence while only degrading resolution by 3.2$\times$. Alternatively, the low-NA design is capable of three-color fluorescence imaging with 110-$μ$m resolution at a 1-mm working distance. Overall, we demonstrate a versatile optical frontend that is adaptable to a range of applications using different fluorophores, illumination configurations, and lensless imaging techniques. |
| title | A Versatile Optical Frontend for Multicolor Fluorescence Imaging with Miniaturized Lensless Sensors |
| topic | Image and Video Processing |
| url | https://arxiv.org/abs/2511.17860 |