Enumeration of Autocatalytic Subsystems in Large Chemical Reaction Networks

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Main Authors: Golnik, Richard, Gatter, Thomas, Stadler, Peter F., Vassena, Nicola
Format: Preprint
Published: 2025
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_version_ 1866915978480189440
author Golnik, Richard
Gatter, Thomas
Stadler, Peter F.
Vassena, Nicola
author_facet Golnik, Richard
Gatter, Thomas
Stadler, Peter F.
Vassena, Nicola
contents Autocatalysis is an important feature of metabolic networks, contributing crucially to the self-maintenance of organisms. Autocatalytic subsystems of chemical reaction networks (CRNs) are characterized in terms of algebraic conditions on submatrices of the stoichiometric matrix. Here, we derive sufficient conditions for subgraphs supporting irreducible autocatalytic systems in the bipartite Kőnig representation of the CRN. On this basis, we develop an efficient algorithm to enumerate autocatalytic subnetworks and, as a special case, autocatalytic cores, i.e., minimal autocatalytic subnetworks, in full-size metabolic networks. The same algorithmic approach can also be used to determine autocatalytic cores only. As a showcase application, we provide a complete analysis of autocatalysis in the core metabolism of E. coli and enumerate irreducible autocatalytic subsystems of limited size in full-fledged metabolic networks of E. coli, human erythrocytes, and Methanosarcina barkeri (Archea). The mathematical and algorithmic results are accompanied by software enabling the routine analysis of autocatalysis in large CRNs.
format Preprint
id arxiv_https___arxiv_org_abs_2511_18883
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Enumeration of Autocatalytic Subsystems in Large Chemical Reaction Networks
Golnik, Richard
Gatter, Thomas
Stadler, Peter F.
Vassena, Nicola
Molecular Networks
Combinatorics
Cell Behavior
92C42, 68R10, 05C65
Autocatalysis is an important feature of metabolic networks, contributing crucially to the self-maintenance of organisms. Autocatalytic subsystems of chemical reaction networks (CRNs) are characterized in terms of algebraic conditions on submatrices of the stoichiometric matrix. Here, we derive sufficient conditions for subgraphs supporting irreducible autocatalytic systems in the bipartite Kőnig representation of the CRN. On this basis, we develop an efficient algorithm to enumerate autocatalytic subnetworks and, as a special case, autocatalytic cores, i.e., minimal autocatalytic subnetworks, in full-size metabolic networks. The same algorithmic approach can also be used to determine autocatalytic cores only. As a showcase application, we provide a complete analysis of autocatalysis in the core metabolism of E. coli and enumerate irreducible autocatalytic subsystems of limited size in full-fledged metabolic networks of E. coli, human erythrocytes, and Methanosarcina barkeri (Archea). The mathematical and algorithmic results are accompanied by software enabling the routine analysis of autocatalysis in large CRNs.
title Enumeration of Autocatalytic Subsystems in Large Chemical Reaction Networks
topic Molecular Networks
Combinatorics
Cell Behavior
92C42, 68R10, 05C65
url https://arxiv.org/abs/2511.18883