A software-based focus system for wide-field optical microscopy
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arXiv
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| Hauptverfasser: | , |
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| Format: | Preprint |
| Veröffentlicht: |
2025
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| _version_ | 1866915637967716352 |
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| author | Djafer-Cherif, Ilyas Waclaw, Bartlomiej |
| author_facet | Djafer-Cherif, Ilyas Waclaw, Bartlomiej |
| contents | Long-term time-lapse imaging of biological samples requires correcting for focal drift, which would otherwise gradually push the sample out of focus. We present a software-based method that eliminates this time-dependent blur using only a motorized Z-drive, with no additional hardware. The method relies on imaging marks made on the side of the coverslip opposite to the sample. We provide a Beanshell script implementation, evaluate its performance across multiple objectives, and benchmark it against a hardware autofocus system, finding comparable results. Finally, we demonstrate its effectiveness in live imaging of growing bacterial colonies. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2511_20890 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | A software-based focus system for wide-field optical microscopy Djafer-Cherif, Ilyas Waclaw, Bartlomiej Biological Physics Quantitative Methods Long-term time-lapse imaging of biological samples requires correcting for focal drift, which would otherwise gradually push the sample out of focus. We present a software-based method that eliminates this time-dependent blur using only a motorized Z-drive, with no additional hardware. The method relies on imaging marks made on the side of the coverslip opposite to the sample. We provide a Beanshell script implementation, evaluate its performance across multiple objectives, and benchmark it against a hardware autofocus system, finding comparable results. Finally, we demonstrate its effectiveness in live imaging of growing bacterial colonies. |
| title | A software-based focus system for wide-field optical microscopy |
| topic | Biological Physics Quantitative Methods |
| url | https://arxiv.org/abs/2511.20890 |