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| Auteurs principaux: | , , , , |
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| Format: | Preprint |
| Publié: |
2025
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| Sujets: | |
| Accès en ligne: | https://arxiv.org/abs/2512.00225 |
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| _version_ | 1866911294117904384 |
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| author | Czuchnowski, Jakub Li, Chuan Ni, Hongli Weissbourd, Brandon Mertz, Jerome |
| author_facet | Czuchnowski, Jakub Li, Chuan Ni, Hongli Weissbourd, Brandon Mertz, Jerome |
| contents | Deconvolution is the most widely used aberration correction technique in microscopy, however most techniques assume that the aberrations are the same for each point in the image, which is rarely true. Methods for tracking spatially varying aberrations require burdensome calibration or computation, or require symmetries in the aberration patterns. Here, we expand on existing modal deconvolution methods to demonstrate 3D fluorescence deconvolution in imaging systems that exhibit no simple symmetry. Our method is based on a space-variant generalization of Richardson-Lucy deconvolution that makes use of ZEMAX\textsuperscript{\textregistered}-derived point spread functions without the requirement of guide stars or calibration measurements. We validate the performance of our method by applying it to snapshot multiplane imaging of both bead samples and biological specimens, and show that modal decomposition is a practical solution for deconvolving spatially varying aberrations that do not display clear symmetries. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2512_00225 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | 3D space-variant modal deconvolution with computed point spread functions Czuchnowski, Jakub Li, Chuan Ni, Hongli Weissbourd, Brandon Mertz, Jerome Optics Deconvolution is the most widely used aberration correction technique in microscopy, however most techniques assume that the aberrations are the same for each point in the image, which is rarely true. Methods for tracking spatially varying aberrations require burdensome calibration or computation, or require symmetries in the aberration patterns. Here, we expand on existing modal deconvolution methods to demonstrate 3D fluorescence deconvolution in imaging systems that exhibit no simple symmetry. Our method is based on a space-variant generalization of Richardson-Lucy deconvolution that makes use of ZEMAX\textsuperscript{\textregistered}-derived point spread functions without the requirement of guide stars or calibration measurements. We validate the performance of our method by applying it to snapshot multiplane imaging of both bead samples and biological specimens, and show that modal decomposition is a practical solution for deconvolving spatially varying aberrations that do not display clear symmetries. |
| title | 3D space-variant modal deconvolution with computed point spread functions |
| topic | Optics |
| url | https://arxiv.org/abs/2512.00225 |