Near-infrared fluorescent nanoprobes for irreversibility in nonequilibrium actomyosin networks
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| Format: | Preprint |
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2025
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| _version_ | 1866912751069167616 |
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| author | Hendler-Neumark, Adi Magar, Itamar Kleiner, Shirel Rosenberg, Geffen Bisker, Gili |
| author_facet | Hendler-Neumark, Adi Magar, Itamar Kleiner, Shirel Rosenberg, Geffen Bisker, Gili |
| contents | Actomyosin networks operate far from equilibrium, yet detecting the onset of motor-driven irreversible dynamics remains challenging. Here, we embed near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWCNTs) within reconstituted actin networks, and use their nonphotobleaching emission to optically report ATP-powered myosin contractile activity. G-actin-dispersed SWCNTs are incorporated into polymerized F-actin without perturbing network assembly, enabling long-term, single-emitter fluorescence monitoring. Upon myosin addition, the NIR fluorescence levels of individual SWCNTs exhibit enhanced temporal fluctuations, and population-level statistics reveal deviations from equilibrium behaviour. The index of dispersion (IOD) distributions shift and broaden relative to equilibrium baselines, and the Kullback-Leibler divergence between IOD distributions systematically increases with increasing motor activity. Stationarity analysis further shows a dose-dependent increase in the fraction of nonstationary fluorescence traces, indicating the emergence of irreversible, time-evolving dynamics. These results establish SWCNTs as minimally invasive optical probes of irreversibility in nonequilibrium actomyosin assemblies, with broad applicability to other active biopolymer systems. |
| format | Preprint |
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arxiv_https___arxiv_org_abs_2512_05443 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Near-infrared fluorescent nanoprobes for irreversibility in nonequilibrium actomyosin networks Hendler-Neumark, Adi Magar, Itamar Kleiner, Shirel Rosenberg, Geffen Bisker, Gili Biological Physics Actomyosin networks operate far from equilibrium, yet detecting the onset of motor-driven irreversible dynamics remains challenging. Here, we embed near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWCNTs) within reconstituted actin networks, and use their nonphotobleaching emission to optically report ATP-powered myosin contractile activity. G-actin-dispersed SWCNTs are incorporated into polymerized F-actin without perturbing network assembly, enabling long-term, single-emitter fluorescence monitoring. Upon myosin addition, the NIR fluorescence levels of individual SWCNTs exhibit enhanced temporal fluctuations, and population-level statistics reveal deviations from equilibrium behaviour. The index of dispersion (IOD) distributions shift and broaden relative to equilibrium baselines, and the Kullback-Leibler divergence between IOD distributions systematically increases with increasing motor activity. Stationarity analysis further shows a dose-dependent increase in the fraction of nonstationary fluorescence traces, indicating the emergence of irreversible, time-evolving dynamics. These results establish SWCNTs as minimally invasive optical probes of irreversibility in nonequilibrium actomyosin assemblies, with broad applicability to other active biopolymer systems. |
| title | Near-infrared fluorescent nanoprobes for irreversibility in nonequilibrium actomyosin networks |
| topic | Biological Physics |
| url | https://arxiv.org/abs/2512.05443 |