Saved in:
Bibliographic Details
Main Authors: Gu, Jeffrey, Jeon, Minkyu, Ma, Ambri, Yeung-Levy, Serena, Zhong, Ellen D.
Format: Preprint
Published: 2025
Subjects:
Online Access:https://arxiv.org/abs/2512.06332
Tags: Add Tag
No Tags, Be the first to tag this record!
Table of Contents:
  • Cryo-electron microscopy (cryo-EM) is an indispensable technique for determining the 3D structures of dynamic biomolecular complexes. While typically applied to image a single molecular species, cryo-EM has the potential for structure determination of many targets simultaneously in a high-throughput fashion. However, existing methods typically focus on modeling conformational heterogeneity within a single or a few structures and are not designed to resolve compositional heterogeneity arising from mixtures of many distinct molecular species. To address this challenge, we propose CryoHype, a transformer-based hypernetwork for cryo-EM reconstruction that dynamically adjusts the weights of an implicit neural representation. Using CryoHype, we achieve state-of-the-art results on a challenging benchmark dataset containing 100 structures. We further demonstrate that CryoHype scales to the reconstruction of 1,000 distinct structures from unlabeled cryo-EM images in the fixed-pose setting.