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Main Authors: Wei, Sibo, Chen, Peng, Dong, Lifeng, Luo, Yin, Wang, Lei, Zhang, Peng, Lu, Wenpeng, Guo, Jianbin, Yang, Hongjun, Zeng, Dajun
Format: Preprint
Published: 2025
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Online Access:https://arxiv.org/abs/2512.24733
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author Wei, Sibo
Chen, Peng
Dong, Lifeng
Luo, Yin
Wang, Lei
Zhang, Peng
Lu, Wenpeng
Guo, Jianbin
Yang, Hongjun
Zeng, Dajun
author_facet Wei, Sibo
Chen, Peng
Dong, Lifeng
Luo, Yin
Wang, Lei
Zhang, Peng
Lu, Wenpeng
Guo, Jianbin
Yang, Hongjun
Zeng, Dajun
contents Multi-omics studies often rely on pathway enrichment to interpret heterogeneous molecular changes, but pathway enrichment (PE)-based workflows inherit structural limitations of pathway resources, including curation lag, functional redundancy, and limited sensitivity to molecular states and interventions. Although recent work has explored using large language models (LLMs) to improve PE-based interpretation, the lack of a standardized benchmark for end-to-end multi-omics pathway mechanism elucidation has largely confined evaluation to small, manually curated datasets or ad hoc case studies, hindering reproducible progress. To address this issue, we introduce BIOME-Bench, constructed via a rigorous four-stage workflow, to evaluate two core capabilities of LLMs in multi-omics analysis: Biomolecular Interaction Inference and end-to-end Multi-Omics Pathway Mechanism Elucidation. We develop evaluation protocols for both tasks and conduct comprehensive experiments across multiple strong contemporary models. Experimental results demonstrate that existing models still exhibit substantial deficiencies in multi-omics analysis, struggling to reliably distinguish fine-grained biomolecular relation types and to generate faithful, robust pathway-level mechanistic explanations.
format Preprint
id arxiv_https___arxiv_org_abs_2512_24733
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle BIOME-Bench: A Benchmark for Biomolecular Interaction Inference and Multi-Omics Pathway Mechanism Elucidation from Scientific Literature
Wei, Sibo
Chen, Peng
Dong, Lifeng
Luo, Yin
Wang, Lei
Zhang, Peng
Lu, Wenpeng
Guo, Jianbin
Yang, Hongjun
Zeng, Dajun
Computation and Language
Multi-omics studies often rely on pathway enrichment to interpret heterogeneous molecular changes, but pathway enrichment (PE)-based workflows inherit structural limitations of pathway resources, including curation lag, functional redundancy, and limited sensitivity to molecular states and interventions. Although recent work has explored using large language models (LLMs) to improve PE-based interpretation, the lack of a standardized benchmark for end-to-end multi-omics pathway mechanism elucidation has largely confined evaluation to small, manually curated datasets or ad hoc case studies, hindering reproducible progress. To address this issue, we introduce BIOME-Bench, constructed via a rigorous four-stage workflow, to evaluate two core capabilities of LLMs in multi-omics analysis: Biomolecular Interaction Inference and end-to-end Multi-Omics Pathway Mechanism Elucidation. We develop evaluation protocols for both tasks and conduct comprehensive experiments across multiple strong contemporary models. Experimental results demonstrate that existing models still exhibit substantial deficiencies in multi-omics analysis, struggling to reliably distinguish fine-grained biomolecular relation types and to generate faithful, robust pathway-level mechanistic explanations.
title BIOME-Bench: A Benchmark for Biomolecular Interaction Inference and Multi-Omics Pathway Mechanism Elucidation from Scientific Literature
topic Computation and Language
url https://arxiv.org/abs/2512.24733