Wetting-coupled phase separation as an energetic mechanism for active bacterial adhesion

Fuente: arXiv
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Auteurs principaux: Yang, Dixi, Wang, Anheng, Huang, Jia, Zhuo, Xiaofeng, Wang, Chunming, Tanaka, Hajime, Yuan, Jiaxing
Format: Preprint
Publié: 2026
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author Yang, Dixi
Wang, Anheng
Huang, Jia
Zhuo, Xiaofeng
Wang, Chunming
Tanaka, Hajime
Yuan, Jiaxing
author_facet Yang, Dixi
Wang, Anheng
Huang, Jia
Zhuo, Xiaofeng
Wang, Chunming
Tanaka, Hajime
Yuan, Jiaxing
contents The rapid adhesion of motile bacteria from dilute suspensions poses a fundamental non-equilibrium problem: hydrodynamic interactions bias bacterial motion near surfaces without generating stable confinement, while electrostatic interactions are predominantly repulsive. Here, combining experiments on Pseudomonas aeruginosa and Staphylococcus aureus in a polyethylene glycol/dextran aqueous two-phase system with large-scale hydrodynamic simulations, we identify wetting-coupled liquid--liquid phase separation (LLPS) as an energetic trapping mechanism for bacterial adhesion. When bacteria partition into a phase that preferentially wets the substrate, interfacial free-energy minimization creates a deep energetic trap that stabilizes adhesion and induces lateral clustering via capillary interactions. Crucially, bacterial motility plays a dual role: at low phase volume fractions, activity enhances transport into the wetting layer and promotes accumulation, whereas at higher phase volumes it suppresses adhesion through the formation of self-spinning droplets that generate hydrodynamic lift opposing interfacial trapping. Our results establish wetting-coupled LLPS as a generic physical route governing interfacial organization in active suspensions. This provides a unified energetic framework for bacterial adhesion in complex fluids, with broad implications for deciphering bacterial-cell interactions and controlling biofilm formation.
format Preprint
id arxiv_https___arxiv_org_abs_2601_06754
institution arXiv
publishDate 2026
record_format arxiv
spellingShingle Wetting-coupled phase separation as an energetic mechanism for active bacterial adhesion
Yang, Dixi
Wang, Anheng
Huang, Jia
Zhuo, Xiaofeng
Wang, Chunming
Tanaka, Hajime
Yuan, Jiaxing
Soft Condensed Matter
The rapid adhesion of motile bacteria from dilute suspensions poses a fundamental non-equilibrium problem: hydrodynamic interactions bias bacterial motion near surfaces without generating stable confinement, while electrostatic interactions are predominantly repulsive. Here, combining experiments on Pseudomonas aeruginosa and Staphylococcus aureus in a polyethylene glycol/dextran aqueous two-phase system with large-scale hydrodynamic simulations, we identify wetting-coupled liquid--liquid phase separation (LLPS) as an energetic trapping mechanism for bacterial adhesion. When bacteria partition into a phase that preferentially wets the substrate, interfacial free-energy minimization creates a deep energetic trap that stabilizes adhesion and induces lateral clustering via capillary interactions. Crucially, bacterial motility plays a dual role: at low phase volume fractions, activity enhances transport into the wetting layer and promotes accumulation, whereas at higher phase volumes it suppresses adhesion through the formation of self-spinning droplets that generate hydrodynamic lift opposing interfacial trapping. Our results establish wetting-coupled LLPS as a generic physical route governing interfacial organization in active suspensions. This provides a unified energetic framework for bacterial adhesion in complex fluids, with broad implications for deciphering bacterial-cell interactions and controlling biofilm formation.
title Wetting-coupled phase separation as an energetic mechanism for active bacterial adhesion
topic Soft Condensed Matter
url https://arxiv.org/abs/2601.06754