Three-Dimensional Volumetric Reconstruction of Native Chilean Pollen via Lens-Free Digital In-line Holographic Microscopy
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| Format: | Preprint |
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2026
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| author | Staforelli-Vivanco, J. Salamanca-Levi, V. Jofré-Cerda, R. Rondanelli-Reyes, M. Lamas, I. |
| author_facet | Staforelli-Vivanco, J. Salamanca-Levi, V. Jofré-Cerda, R. Rondanelli-Reyes, M. Lamas, I. |
| contents | This study presents a robust methodology for the three-dimensional (3D) volumetric reconstruction and morphological characterization of native Chilean pollen grains using a lens-free Digital In-line Holographic Microscopy (DLHM) system. Utilizing a 532 nm laser point-source configuration and a 3.45 $μ$m pixel pitch CMOS sensor , we achieved a geometric magnification of 50x, resulting in an effective lateral resolution of approximately 69 nm at the object plane. The complex wavefronts of \textit{Anthemis cotula} (chamomile), \textit{Gevuina avellana} (hazel), and \textit{Conium maculatum} (hemlock) were numerically reconstructed via the Kirchhoff-Helmholtz transform to generate high-fidelity 3D refractive index maps. Biophysical parameters were extracted with nanometric precision, with volumes ranging from $3780.2 \pm 18$ $μ$m$^3$ to $4320.5 \pm 15$ $μ$m$^3$. Morphological quantification identified \textit{A. cotula} as the least spherical species ($Ψ= 0.76 \pm 0.03$) due to its characteristic echinate (spiny) exine, while \textit{G. avellana} exhibited the highest sphericity index of $0.89 \pm 0.02$. These results demonstrate that the label-free retrieval of "digital fingerprints" provides a scalable alternative for automated melissopalynology and viability assessment, filling critical geographic data gaps in South American biodiversity hotspots. |
| format | Preprint |
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arxiv_https___arxiv_org_abs_2601_14205 |
| institution | arXiv |
| publishDate | 2026 |
| record_format | arxiv |
| spellingShingle | Three-Dimensional Volumetric Reconstruction of Native Chilean Pollen via Lens-Free Digital In-line Holographic Microscopy Staforelli-Vivanco, J. Salamanca-Levi, V. Jofré-Cerda, R. Rondanelli-Reyes, M. Lamas, I. Optics Biological Physics Quantitative Methods This study presents a robust methodology for the three-dimensional (3D) volumetric reconstruction and morphological characterization of native Chilean pollen grains using a lens-free Digital In-line Holographic Microscopy (DLHM) system. Utilizing a 532 nm laser point-source configuration and a 3.45 $μ$m pixel pitch CMOS sensor , we achieved a geometric magnification of 50x, resulting in an effective lateral resolution of approximately 69 nm at the object plane. The complex wavefronts of \textit{Anthemis cotula} (chamomile), \textit{Gevuina avellana} (hazel), and \textit{Conium maculatum} (hemlock) were numerically reconstructed via the Kirchhoff-Helmholtz transform to generate high-fidelity 3D refractive index maps. Biophysical parameters were extracted with nanometric precision, with volumes ranging from $3780.2 \pm 18$ $μ$m$^3$ to $4320.5 \pm 15$ $μ$m$^3$. Morphological quantification identified \textit{A. cotula} as the least spherical species ($Ψ= 0.76 \pm 0.03$) due to its characteristic echinate (spiny) exine, while \textit{G. avellana} exhibited the highest sphericity index of $0.89 \pm 0.02$. These results demonstrate that the label-free retrieval of "digital fingerprints" provides a scalable alternative for automated melissopalynology and viability assessment, filling critical geographic data gaps in South American biodiversity hotspots. |
| title | Three-Dimensional Volumetric Reconstruction of Native Chilean Pollen via Lens-Free Digital In-line Holographic Microscopy |
| topic | Optics Biological Physics Quantitative Methods |
| url | https://arxiv.org/abs/2601.14205 |