Feasibility of Concurrent 1H MRS & 31P MRSI at 7T: Brain Energy Metabolism Responses to Hyperglycemia

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Main Authors: Widmaier, Mark, Matson, Brooke C., Emir, Uzay, Hwang, Janice J.
Format: Preprint
Published: 2026
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author Widmaier, Mark
Matson, Brooke C.
Emir, Uzay
Hwang, Janice J.
author_facet Widmaier, Mark
Matson, Brooke C.
Emir, Uzay
Hwang, Janice J.
contents How the human brain adjusts fuel handling and its bioenergetic state during changing glucose levels remains difficult to assess noninvasively. In this study, we established an interleaved multinuclear 7 T MR spectroscopy protocol to track a glucose-related 1H signal alongside 31P measures of high-energy phosphate metabolism during a hyperglycemic clamp. Five healthy adults completed a morning, fasted infusion experiment consisting of baseline, ramp-up, and hyperglycemic stages over approx. 120 min. Short-block, short-TE 1H single-voxel spectroscopy (STEAM, TE = 11 ms; mean block duration 5.71 (SD:0.62 min)) was acquired in frontal cortex and quantified using the composite Glucose+Taurine (Glc+Tau) measure. 31P was acquired with rapid 3D PETALUTE MRSI using an ultrashort echo time (UTE; TE = 65 micros; 381 s per block), and high-energy phosphate ratios were derived from a posterior cortical region of interest. Across participants, 1H Glc+Tau increased with blood glucose and showed significant elevations from baseline into hyperglycemia. In parallel, 31P ratios exhibited smaller but significant glycemia-linked responses: both PCr/Pi and gamma-ATP/Pi increased with blood glucose and differed across glucose clamp stages. These findings show that 1H and 31P MRS(I) can be interleaved at 7 T to measure energy metabolism within a single session
format Preprint
id arxiv_https___arxiv_org_abs_2603_08829
institution arXiv
publishDate 2026
record_format arxiv
spellingShingle Feasibility of Concurrent 1H MRS & 31P MRSI at 7T: Brain Energy Metabolism Responses to Hyperglycemia
Widmaier, Mark
Matson, Brooke C.
Emir, Uzay
Hwang, Janice J.
Medical Physics
How the human brain adjusts fuel handling and its bioenergetic state during changing glucose levels remains difficult to assess noninvasively. In this study, we established an interleaved multinuclear 7 T MR spectroscopy protocol to track a glucose-related 1H signal alongside 31P measures of high-energy phosphate metabolism during a hyperglycemic clamp. Five healthy adults completed a morning, fasted infusion experiment consisting of baseline, ramp-up, and hyperglycemic stages over approx. 120 min. Short-block, short-TE 1H single-voxel spectroscopy (STEAM, TE = 11 ms; mean block duration 5.71 (SD:0.62 min)) was acquired in frontal cortex and quantified using the composite Glucose+Taurine (Glc+Tau) measure. 31P was acquired with rapid 3D PETALUTE MRSI using an ultrashort echo time (UTE; TE = 65 micros; 381 s per block), and high-energy phosphate ratios were derived from a posterior cortical region of interest. Across participants, 1H Glc+Tau increased with blood glucose and showed significant elevations from baseline into hyperglycemia. In parallel, 31P ratios exhibited smaller but significant glycemia-linked responses: both PCr/Pi and gamma-ATP/Pi increased with blood glucose and differed across glucose clamp stages. These findings show that 1H and 31P MRS(I) can be interleaved at 7 T to measure energy metabolism within a single session
title Feasibility of Concurrent 1H MRS & 31P MRSI at 7T: Brain Energy Metabolism Responses to Hyperglycemia
topic Medical Physics
url https://arxiv.org/abs/2603.08829