Widefield Nanodiamond Quantum Sensing Based on Light-Sheet Microscopy
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arXiv
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| Format: | Preprint |
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2026
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| author | Wang, Shuo Ai, Ming-Zhong Fan, Jing-Wei Ye, Junchen Lin, Chao Li, Quan Liu, Ren-Bao |
| author_facet | Wang, Shuo Ai, Ming-Zhong Fan, Jing-Wei Ye, Junchen Lin, Chao Li, Quan Liu, Ren-Bao |
| contents | Nanodiamonds containing nitrogen-vacancy (NV) centers are promising quantum sensors for biological applications thanks to their sub-micron spatial resolution, biocompatibility, and versatile multi-modal responses. However, the optically detected magnetic resonance (ODMR) measurement requires laser irradiation, creating a trade-off between high-throughput and low phototoxicity for applications in live cells. Here to address this challenge we develop a widefield quantum sensing method based on light-sheet microscopy (LSM), in which the sample is illuminated by a vertically movable laser sheet and the fluorescence is collected along the vertical axis that is orthogonal to the light sheet. This LSM-ODMR system is demonstrated to feature high throughput sensing due to the wide-field configuration, fast three-dimensional imaging and sensing due to the vertical mobility of the light sheet, enhanced sensitivity due to suppression of out-of-focus background fluorescence, and low phototoxicity for bio-sensing due to elimination of out-of-focus illumination. This LSM-based widefield nanodiamond sensing provides an approach for biological studies with low phototoxicity, offering three-dimensional and multi-modal sensing capability. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2603_19593 |
| institution | arXiv |
| publishDate | 2026 |
| record_format | arxiv |
| spellingShingle | Widefield Nanodiamond Quantum Sensing Based on Light-Sheet Microscopy Wang, Shuo Ai, Ming-Zhong Fan, Jing-Wei Ye, Junchen Lin, Chao Li, Quan Liu, Ren-Bao Applied Physics Optics Nanodiamonds containing nitrogen-vacancy (NV) centers are promising quantum sensors for biological applications thanks to their sub-micron spatial resolution, biocompatibility, and versatile multi-modal responses. However, the optically detected magnetic resonance (ODMR) measurement requires laser irradiation, creating a trade-off between high-throughput and low phototoxicity for applications in live cells. Here to address this challenge we develop a widefield quantum sensing method based on light-sheet microscopy (LSM), in which the sample is illuminated by a vertically movable laser sheet and the fluorescence is collected along the vertical axis that is orthogonal to the light sheet. This LSM-ODMR system is demonstrated to feature high throughput sensing due to the wide-field configuration, fast three-dimensional imaging and sensing due to the vertical mobility of the light sheet, enhanced sensitivity due to suppression of out-of-focus background fluorescence, and low phototoxicity for bio-sensing due to elimination of out-of-focus illumination. This LSM-based widefield nanodiamond sensing provides an approach for biological studies with low phototoxicity, offering three-dimensional and multi-modal sensing capability. |
| title | Widefield Nanodiamond Quantum Sensing Based on Light-Sheet Microscopy |
| topic | Applied Physics Optics |
| url | https://arxiv.org/abs/2603.19593 |