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Main Authors: Zhou, Chenxu, Liu, Zelin, Cai, Rui, Gong, Houlin, Yu, Yikang, Zeng, Jia, Pei, Yanru, Zhang, Liang, Zhao, Weishu, Gao, Xiaofeng
Format: Preprint
Published: 2026
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Online Access:https://arxiv.org/abs/2603.23961
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author Zhou, Chenxu
Liu, Zelin
Cai, Rui
Gong, Houlin
Yu, Yikang
Zeng, Jia
Pei, Yanru
Zhang, Liang
Zhao, Weishu
Gao, Xiaofeng
author_facet Zhou, Chenxu
Liu, Zelin
Cai, Rui
Gong, Houlin
Yu, Yikang
Zeng, Jia
Pei, Yanru
Zhang, Liang
Zhao, Weishu
Gao, Xiaofeng
contents Deep-sea cold seep stage assessment has traditionally relied on costly, high-risk manned submersible operations and visual surveys of macrofauna. Although microbial communities provide a promising and more cost-effective alternative, reliable inference remains challenging because the available deep-sea dataset is extremely small ($n = 13$) relative to the microbial feature dimension ($p = 26$), making purely data-driven models highly prone to overfitting. To address this, we propose a knowledge-enhanced classification framework that incorporates an ecological knowledge graph as a structural prior. By fusing macro-microbe coupling and microbial co-occurrence patterns, the framework internalizes established ecological logic into a \underline{\textbf{G}}raph-\underline{\textbf{R}}egularized \underline{\textbf{M}}ultinomial \underline{\textbf{L}}ogistic \underline{\textbf{R}}egression (GRMLR) model, effectively constraining the feature space through a manifold penalty to ensure biologically consistent classification. Importantly, the framework removes the need for macrofauna observations at inference time: macro-microbe associations are used only to guide training, whereas prediction relies solely on microbial abundance profiles. Experimental results demonstrate that our approach significantly outperforms standard baselines, highlighting its potential as a robust and scalable framework for deep-sea ecological assessment.
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institution arXiv
publishDate 2026
record_format arxiv
spellingShingle GRMLR: Knowledge-Enhanced Small-Data Learning for Deep-Sea Cold Seep Stage Inference
Zhou, Chenxu
Liu, Zelin
Cai, Rui
Gong, Houlin
Yu, Yikang
Zeng, Jia
Pei, Yanru
Zhang, Liang
Zhao, Weishu
Gao, Xiaofeng
Machine Learning
Computer Vision and Pattern Recognition
Deep-sea cold seep stage assessment has traditionally relied on costly, high-risk manned submersible operations and visual surveys of macrofauna. Although microbial communities provide a promising and more cost-effective alternative, reliable inference remains challenging because the available deep-sea dataset is extremely small ($n = 13$) relative to the microbial feature dimension ($p = 26$), making purely data-driven models highly prone to overfitting. To address this, we propose a knowledge-enhanced classification framework that incorporates an ecological knowledge graph as a structural prior. By fusing macro-microbe coupling and microbial co-occurrence patterns, the framework internalizes established ecological logic into a \underline{\textbf{G}}raph-\underline{\textbf{R}}egularized \underline{\textbf{M}}ultinomial \underline{\textbf{L}}ogistic \underline{\textbf{R}}egression (GRMLR) model, effectively constraining the feature space through a manifold penalty to ensure biologically consistent classification. Importantly, the framework removes the need for macrofauna observations at inference time: macro-microbe associations are used only to guide training, whereas prediction relies solely on microbial abundance profiles. Experimental results demonstrate that our approach significantly outperforms standard baselines, highlighting its potential as a robust and scalable framework for deep-sea ecological assessment.
title GRMLR: Knowledge-Enhanced Small-Data Learning for Deep-Sea Cold Seep Stage Inference
topic Machine Learning
Computer Vision and Pattern Recognition
url https://arxiv.org/abs/2603.23961