High-resolution scanning fluorescence imaging through scattering via speckle replica alignment and variance computation
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arXiv
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| Autori principali: | , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2026
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| _version_ | 1866911548366127104 |
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| author | Zhu, Lei Wu, Tengfei Rauer, Bernhard de Aguiar, Hilton B. Gigan, Sylvain |
| author_facet | Zhu, Lei Wu, Tengfei Rauer, Bernhard de Aguiar, Hilton B. Gigan, Sylvain |
| contents | Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational framework that retrieves high-resolution images by aligning local speckle replicas and computing their pixel-wise variance. We demonstrate its versatility in two regimes: linear wide-field one-photon (1P) fluorescence imaging and nonlinear two-photon (2P) fluorescence imaging where the object is excited by a scanned speckle field and detected with a single-pixel detector. This approach outperforms standard autocorrelation techniques in terms of resolution and convergence. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2603_26531 |
| institution | arXiv |
| publishDate | 2026 |
| record_format | arxiv |
| spellingShingle | High-resolution scanning fluorescence imaging through scattering via speckle replica alignment and variance computation Zhu, Lei Wu, Tengfei Rauer, Bernhard de Aguiar, Hilton B. Gigan, Sylvain Optics Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational framework that retrieves high-resolution images by aligning local speckle replicas and computing their pixel-wise variance. We demonstrate its versatility in two regimes: linear wide-field one-photon (1P) fluorescence imaging and nonlinear two-photon (2P) fluorescence imaging where the object is excited by a scanned speckle field and detected with a single-pixel detector. This approach outperforms standard autocorrelation techniques in terms of resolution and convergence. |
| title | High-resolution scanning fluorescence imaging through scattering via speckle replica alignment and variance computation |
| topic | Optics |
| url | https://arxiv.org/abs/2603.26531 |