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Autori principali: Occhicone, Agostino, Sinibaldi, Alberto, Munzert, Peter, Butt, Jordan N., Luta, Ethan P., Arévalo, Diego M., Michelotti, Francesco, Miller, Benjamin L.
Natura: Preprint
Pubblicazione: 2026
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Accesso online:https://arxiv.org/abs/2604.08225
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author Occhicone, Agostino
Sinibaldi, Alberto
Munzert, Peter
Butt, Jordan N.
Luta, Ethan P.
Arévalo, Diego M.
Michelotti, Francesco
Miller, Benjamin L.
author_facet Occhicone, Agostino
Sinibaldi, Alberto
Munzert, Peter
Butt, Jordan N.
Luta, Ethan P.
Arévalo, Diego M.
Michelotti, Francesco
Miller, Benjamin L.
contents This study presents a rigorous comparative analysis of two label-free optical biosensing platforms, Bloch surface wave (BSW) and microring resonator (MRR), for the detection of SARS-CoV-2 antibodies in human serum. To ensure direct comparability, a new BSW readout system was established alongside an existing MRR platform, allowing assays to be conducted under nearly identical experimental conditions. Both sensors were functionalized with various SARS-CoV-2 Spike and Nucleocapsid protein variants to capture specific host antibodies. The results demonstrate that both platforms provide rapid, quantitative, and sensitive detection of anti-Spike and anti-Nucleocapsid antibodies without the need for secondary labels. Furthermore, the platforms show excellent agreement with longitudinal serology benchmarks and high repeatability across different biochip batches. This work establishes both BSW and MRR technologies as powerful, low-cost candidates for next-generation clinical diagnostics and serological surveillance.
format Preprint
id arxiv_https___arxiv_org_abs_2604_08225
institution arXiv
publishDate 2026
record_format arxiv
spellingShingle Comparative performance of three optical biosensing platforms for SARS-CoV-2 antibodies detection in human serum
Occhicone, Agostino
Sinibaldi, Alberto
Munzert, Peter
Butt, Jordan N.
Luta, Ethan P.
Arévalo, Diego M.
Michelotti, Francesco
Miller, Benjamin L.
Optics
Materials Science
Soft Condensed Matter
Biological Physics
This study presents a rigorous comparative analysis of two label-free optical biosensing platforms, Bloch surface wave (BSW) and microring resonator (MRR), for the detection of SARS-CoV-2 antibodies in human serum. To ensure direct comparability, a new BSW readout system was established alongside an existing MRR platform, allowing assays to be conducted under nearly identical experimental conditions. Both sensors were functionalized with various SARS-CoV-2 Spike and Nucleocapsid protein variants to capture specific host antibodies. The results demonstrate that both platforms provide rapid, quantitative, and sensitive detection of anti-Spike and anti-Nucleocapsid antibodies without the need for secondary labels. Furthermore, the platforms show excellent agreement with longitudinal serology benchmarks and high repeatability across different biochip batches. This work establishes both BSW and MRR technologies as powerful, low-cost candidates for next-generation clinical diagnostics and serological surveillance.
title Comparative performance of three optical biosensing platforms for SARS-CoV-2 antibodies detection in human serum
topic Optics
Materials Science
Soft Condensed Matter
Biological Physics
url https://arxiv.org/abs/2604.08225