Background Remover -- an effective tool for processing noisy microscopy images

Fuente: arXiv
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Autori principali: Kilian, Anna, Bilski, Paweł, Sankowska, Małgorzata
Natura: Preprint
Pubblicazione: 2026
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author Kilian, Anna
Bilski, Paweł
Sankowska, Małgorzata
author_facet Kilian, Anna
Bilski, Paweł
Sankowska, Małgorzata
contents Background Remover (BGR) is a novel software tool developed as a plugin to the well-known ImageJ program and designed to address the challenges of analysing fluorescent microscopy images characterized by low signal-to-noise ratios and heterogeneous backgrounds. The used algorithm effectively differentiates between signal and noise pixels, preserving the signal while eliminating noise. This functionality enables the analysis of images with objects of varying intensities, allowing for reliable identification even in low signal-to-noise ratio conditions. Furthermore, BGR offers the capability to determine the intensity of identified objects, enhancing its utility for researchers in the field. The paper describes the algorithm and the program functioning, as well as the carried out tests of its performance. The program is freely downloadable from the website https://kilianna.github.io/background-remover/
format Preprint
id arxiv_https___arxiv_org_abs_2604_25558
institution arXiv
publishDate 2026
record_format arxiv
spellingShingle Background Remover -- an effective tool for processing noisy microscopy images
Kilian, Anna
Bilski, Paweł
Sankowska, Małgorzata
Instrumentation and Detectors
Background Remover (BGR) is a novel software tool developed as a plugin to the well-known ImageJ program and designed to address the challenges of analysing fluorescent microscopy images characterized by low signal-to-noise ratios and heterogeneous backgrounds. The used algorithm effectively differentiates between signal and noise pixels, preserving the signal while eliminating noise. This functionality enables the analysis of images with objects of varying intensities, allowing for reliable identification even in low signal-to-noise ratio conditions. Furthermore, BGR offers the capability to determine the intensity of identified objects, enhancing its utility for researchers in the field. The paper describes the algorithm and the program functioning, as well as the carried out tests of its performance. The program is freely downloadable from the website https://kilianna.github.io/background-remover/
title Background Remover -- an effective tool for processing noisy microscopy images
topic Instrumentation and Detectors
url https://arxiv.org/abs/2604.25558