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Main Authors: Sivaprasad, Sarath, Wang, Hui-Po, Jäckel, Anna-Lisa, Baumann, Jonas, Baumann, Carole, Herrmann, Jennifer, Fritz, Mario
Format: Preprint
Published: 2026
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Online Access:https://arxiv.org/abs/2605.10464
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author Sivaprasad, Sarath
Wang, Hui-Po
Jäckel, Anna-Lisa
Baumann, Jonas
Baumann, Carole
Herrmann, Jennifer
Fritz, Mario
author_facet Sivaprasad, Sarath
Wang, Hui-Po
Jäckel, Anna-Lisa
Baumann, Jonas
Baumann, Carole
Herrmann, Jennifer
Fritz, Mario
contents Zebrafish embryos are a valuable model for drug discovery due to their optical transparency and genetic similarity to humans. However, current evaluations rely on manual inspection, which is costly and labor-intensive. While machine learning offers automation potential, progress is limited by the lack of comprehensive datasets. To address this, we introduce a large-scale dataset of high-resolution microscopic image sequences capturing zebrafish embryonic development under both control conditions and exposure to compounds (3,4-dichloroaniline). This dataset, with expert annotations at fine-grained temporal levels, supports two benchmarking tasks: (1) fertility classification, assessing zebrafish egg viability (130,368 images), and (2) toxicity assessment, detecting malformations induced by toxic exposure over time (55,296 images). Alongside the dataset, we present the first transformer-based baseline model that integrates spatiotemporal features to predict developmental abnormalities at early stages. Experimental results present the model's effectiveness, achieving 98% accuracy in fertility classification and 92% in toxicity assessment. These findings underscore the potential of automated approaches to enhance zebrafish-based toxicity analysis.
format Preprint
id arxiv_https___arxiv_org_abs_2605_10464
institution arXiv
publishDate 2026
record_format arxiv
spellingShingle Automated Detection of Abnormalities in Zebrafish Development
Sivaprasad, Sarath
Wang, Hui-Po
Jäckel, Anna-Lisa
Baumann, Jonas
Baumann, Carole
Herrmann, Jennifer
Fritz, Mario
Computer Vision and Pattern Recognition
Zebrafish embryos are a valuable model for drug discovery due to their optical transparency and genetic similarity to humans. However, current evaluations rely on manual inspection, which is costly and labor-intensive. While machine learning offers automation potential, progress is limited by the lack of comprehensive datasets. To address this, we introduce a large-scale dataset of high-resolution microscopic image sequences capturing zebrafish embryonic development under both control conditions and exposure to compounds (3,4-dichloroaniline). This dataset, with expert annotations at fine-grained temporal levels, supports two benchmarking tasks: (1) fertility classification, assessing zebrafish egg viability (130,368 images), and (2) toxicity assessment, detecting malformations induced by toxic exposure over time (55,296 images). Alongside the dataset, we present the first transformer-based baseline model that integrates spatiotemporal features to predict developmental abnormalities at early stages. Experimental results present the model's effectiveness, achieving 98% accuracy in fertility classification and 92% in toxicity assessment. These findings underscore the potential of automated approaches to enhance zebrafish-based toxicity analysis.
title Automated Detection of Abnormalities in Zebrafish Development
topic Computer Vision and Pattern Recognition
url https://arxiv.org/abs/2605.10464