Hybrid spectral-spatial domain registration for nanometric tracking in digital in-line holographic microscopy
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arXiv
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| Format: | Preprint |
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2026
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| author | Kalinowski, Kamil Rogalski, Mikolaj Arcab, Piotr Wdowiak, Emilia Zdankowski, Piotr Trusiak, Maciej |
| author_facet | Kalinowski, Kamil Rogalski, Mikolaj Arcab, Piotr Wdowiak, Emilia Zdankowski, Piotr Trusiak, Maciej |
| contents | Digital in-line holographic microscopy enables label-free tracking and metrology, but achieving nanometric sub-pixel displacement accuracy over a wide capture range remains challenging. Frequency-domain registration based on the discrete Fourier transform (DFT) is globally stable and tolerant to large shifts, yet it suffers from sub-pixel quantization and interpolation artifacts that limit precision near zero displacement. In contrast, spatial-gradient refinement such as Lucas-Kanade (LK) can reach very high sub-pixel accuracy, but it is strongly initialization-limited and prone to divergence outside a narrow convergence basin. Here we propose a Hybrid Spectral-Spatial Domain (HSSD) framework that resolves this trade-off by combining the global robustness of a DFT-based coarse estimator with LK refinement of the residual motion. The DFT stage provides reliable initialization and substantially extends the capture range, while the LK stage suppresses the precision floor by reducing interpolation and quantization errors characteristic of standalone frequency-domain methods. We validate HSSD using numerical simulations and experiments in a transmission in-line holographic imaging system, achieving nanoprecision displacement measurement and stable tracking across a wide range of displacements and defocusing conditions. This hybrid strategy enables reliable nanometric localization in regimes where standalone DFT or LK methods either fail to converge or saturate in accuracy. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2605_24505 |
| institution | arXiv |
| publishDate | 2026 |
| record_format | arxiv |
| spellingShingle | Hybrid spectral-spatial domain registration for nanometric tracking in digital in-line holographic microscopy Kalinowski, Kamil Rogalski, Mikolaj Arcab, Piotr Wdowiak, Emilia Zdankowski, Piotr Trusiak, Maciej Optics Digital in-line holographic microscopy enables label-free tracking and metrology, but achieving nanometric sub-pixel displacement accuracy over a wide capture range remains challenging. Frequency-domain registration based on the discrete Fourier transform (DFT) is globally stable and tolerant to large shifts, yet it suffers from sub-pixel quantization and interpolation artifacts that limit precision near zero displacement. In contrast, spatial-gradient refinement such as Lucas-Kanade (LK) can reach very high sub-pixel accuracy, but it is strongly initialization-limited and prone to divergence outside a narrow convergence basin. Here we propose a Hybrid Spectral-Spatial Domain (HSSD) framework that resolves this trade-off by combining the global robustness of a DFT-based coarse estimator with LK refinement of the residual motion. The DFT stage provides reliable initialization and substantially extends the capture range, while the LK stage suppresses the precision floor by reducing interpolation and quantization errors characteristic of standalone frequency-domain methods. We validate HSSD using numerical simulations and experiments in a transmission in-line holographic imaging system, achieving nanoprecision displacement measurement and stable tracking across a wide range of displacements and defocusing conditions. This hybrid strategy enables reliable nanometric localization in regimes where standalone DFT or LK methods either fail to converge or saturate in accuracy. |
| title | Hybrid spectral-spatial domain registration for nanometric tracking in digital in-line holographic microscopy |
| topic | Optics |
| url | https://arxiv.org/abs/2605.24505 |