Motion-guided sparse correction enables expert-quality point tracking across diverse microscopy regimes

Fuente: arXiv
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Autori principali: Zimianitis, Leonidas, Thenahandi, Pasindu, Buckhalter, Kai, Jayakody, Dineth, Kimura, Julian O., Liang, Xinyue, Cunningham, Karen, Ahmad, Azeem, Ahluwalia, Balpreet S., Jayarathna, Sampath, Chrisochoides, Nikos, Weissbourd, Brandon, Wadduwage, Dushan N.
Natura: Preprint
Pubblicazione: 2026
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author Zimianitis, Leonidas
Thenahandi, Pasindu
Buckhalter, Kai
Jayakody, Dineth
Kimura, Julian O.
Liang, Xinyue
Cunningham, Karen
Ahmad, Azeem
Ahluwalia, Balpreet S.
Jayarathna, Sampath
Chrisochoides, Nikos
Weissbourd, Brandon
Wadduwage, Dushan N.
author_facet Zimianitis, Leonidas
Thenahandi, Pasindu
Buckhalter, Kai
Jayakody, Dineth
Kimura, Julian O.
Liang, Xinyue
Cunningham, Karen
Ahmad, Azeem
Ahluwalia, Balpreet S.
Jayarathna, Sampath
Chrisochoides, Nikos
Weissbourd, Brandon
Wadduwage, Dushan N.
contents Tracking the dynamics of non-canonical biological systems in microscopy videos remains a persistent challenge. Both classical and learning-based trackers depend on expert-reviewed data to be evaluated and adapted, yet exhaustive manual annotation rarely scales to the videos where these tools are needed most. We developed RIPPLE (Refinement Interpolation Platform for Point Location Estimation), which recasts annotation as sparse correction: a user clicks a starting point, RIPPLE proposes a full trajectory, and the user intervenes only where the trajectory drifts. We tested RIPPLE on five challenging microscopy datasets from our laboratories, four from the transparent jellyfish Clytia hemisphaerica and one tracking landmarks on rapidly moving sperm. Across these, RIPPLE matched the quality of exhaustive manual annotation while reducing manual clicks by 3 to 25 times across datasets. RIPPLE thereby fills a missing layer between manual annotation and fully automated tracking, enabling immediate quantification of biological dynamics, method benchmarking, and the production of the gold-standard data needed to adapt future automated microscopy trackers.
format Preprint
id arxiv_https___arxiv_org_abs_2605_29220
institution arXiv
publishDate 2026
record_format arxiv
spellingShingle Motion-guided sparse correction enables expert-quality point tracking across diverse microscopy regimes
Zimianitis, Leonidas
Thenahandi, Pasindu
Buckhalter, Kai
Jayakody, Dineth
Kimura, Julian O.
Liang, Xinyue
Cunningham, Karen
Ahmad, Azeem
Ahluwalia, Balpreet S.
Jayarathna, Sampath
Chrisochoides, Nikos
Weissbourd, Brandon
Wadduwage, Dushan N.
Computer Vision and Pattern Recognition
Tracking the dynamics of non-canonical biological systems in microscopy videos remains a persistent challenge. Both classical and learning-based trackers depend on expert-reviewed data to be evaluated and adapted, yet exhaustive manual annotation rarely scales to the videos where these tools are needed most. We developed RIPPLE (Refinement Interpolation Platform for Point Location Estimation), which recasts annotation as sparse correction: a user clicks a starting point, RIPPLE proposes a full trajectory, and the user intervenes only where the trajectory drifts. We tested RIPPLE on five challenging microscopy datasets from our laboratories, four from the transparent jellyfish Clytia hemisphaerica and one tracking landmarks on rapidly moving sperm. Across these, RIPPLE matched the quality of exhaustive manual annotation while reducing manual clicks by 3 to 25 times across datasets. RIPPLE thereby fills a missing layer between manual annotation and fully automated tracking, enabling immediate quantification of biological dynamics, method benchmarking, and the production of the gold-standard data needed to adapt future automated microscopy trackers.
title Motion-guided sparse correction enables expert-quality point tracking across diverse microscopy regimes
topic Computer Vision and Pattern Recognition
url https://arxiv.org/abs/2605.29220