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| Autores principales: | , , , , , |
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| Formato: | Preprint |
| Publicado: |
2026
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| Materias: | |
| Acceso en línea: | https://arxiv.org/abs/2606.00391 |
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| _version_ | 1866911735073472512 |
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| author | Sarmah, Nipon Finnigan, Tim Taylor, Mark Vinestock, Tom Errington, Ethan Guo, Miao |
| author_facet | Sarmah, Nipon Finnigan, Tim Taylor, Mark Vinestock, Tom Errington, Ethan Guo, Miao |
| contents | Fermentation-derived side streams represent an underutilised resource for sustainable protein production. This study investigates the potential of centrate from industrial Fusarium venenatum fermentation as a nutrient source for fungal biomass generation. Following compositional characterisation, a synthetic centrate medium was formulated and evaluated using a Box-Behnken design combined with response surface methodology. Across 46 experimental runs, cell dry weight (CDW) ranged from 0.22 to 3.87 g per liter, demonstrating a strong dependence on nutrient composition. Ammonia and glucose were identified as the dominant factors influencing biomass production, with significant nonlinear effects. The model predicted a maximum CDW of 4.17 g per liter under optimised conditions, which was experimentally validated at 3.99 g per liter. Carbon conversion efficiency reached up to 29.02%, indicating effective substrate utilisation. These findings demonstrate that fermentation-derived centrate can support substantial fungal growth, while highlighting its potential to enhance nutrient recovery and influence the biochemical composition of sustainable mycoprotein. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2606_00391 |
| institution | arXiv |
| publishDate | 2026 |
| record_format | arxiv |
| spellingShingle | Valorisation of Fermentation Side-Stream for Waste-to-Mycoprotein: Nutrient Composition, Metabolic Insights and Process Optimisation Sarmah, Nipon Finnigan, Tim Taylor, Mark Vinestock, Tom Errington, Ethan Guo, Miao Systems and Control Fermentation-derived side streams represent an underutilised resource for sustainable protein production. This study investigates the potential of centrate from industrial Fusarium venenatum fermentation as a nutrient source for fungal biomass generation. Following compositional characterisation, a synthetic centrate medium was formulated and evaluated using a Box-Behnken design combined with response surface methodology. Across 46 experimental runs, cell dry weight (CDW) ranged from 0.22 to 3.87 g per liter, demonstrating a strong dependence on nutrient composition. Ammonia and glucose were identified as the dominant factors influencing biomass production, with significant nonlinear effects. The model predicted a maximum CDW of 4.17 g per liter under optimised conditions, which was experimentally validated at 3.99 g per liter. Carbon conversion efficiency reached up to 29.02%, indicating effective substrate utilisation. These findings demonstrate that fermentation-derived centrate can support substantial fungal growth, while highlighting its potential to enhance nutrient recovery and influence the biochemical composition of sustainable mycoprotein. |
| title | Valorisation of Fermentation Side-Stream for Waste-to-Mycoprotein: Nutrient Composition, Metabolic Insights and Process Optimisation |
| topic | Systems and Control |
| url | https://arxiv.org/abs/2606.00391 |