Fighting Tuberculosis in an Undergraduate Laboratory: Synthesizing, Evaluating and Analyzing Inhibitors
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ERIC Institute of Education Sciences
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| Main Authors: | , , , |
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| Format: | Recurso educativo Open Access |
| Language: | en |
| Published: |
2015
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| _version_ | 1867181740758401024 |
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| author | Daniels, David Berkes, Charlotte Nekoie, Arjan Franco, Jimmy |
| author_facet | Daniels, David Berkes, Charlotte Nekoie, Arjan Franco, Jimmy Daniels, David Berkes, Charlotte Nekoie, Arjan Franco, Jimmy |
| collection | Education Resources Information Center |
| contents | Fighting Tuberculosis in an Undergraduate Laboratory: Synthesizing, Evaluating and Analyzing Inhibitors Daniels, David Berkes, Charlotte Nekoie, Arjan Franco, Jimmy Communicable Diseases Organic Chemistry Biochemistry College Science Science Instruction Drug Therapy Undergraduate Students Science Laboratories Science Experiments Science Activities Student Projects Interdisciplinary Approach Computer Uses in Education Laboratory Experiments A drug discovery project has been successfully implemented in a first-year general, organic, and biochemistry (GOB) health science course and second-year organic undergraduate chemistry course. This project allows students to apply the fundamental principles of chemistry and biology to a problem of medical significance, practice basic laboratory skills, and understand the interdisciplinary aspect of the drug discovery platform. Students collectively synthesize a small library of antituberculosis compounds and subsequently screen them using a Kirby-Bauer disc diffusion assay for inhibitory activity against "Mycobacterium smegmatis", a known safe surrogate of "Mycobacterium" tuberculosis, the causative agent of tuberculosis. The last unit of the project involves students using PyMOL, a free computer program, to examine a cocrystal structure of the activated inhibitor-enzyme complex, allowing students to identify the molecular interactions responsible for the binding affinity. |
| format | Recurso educativo Open Access |
| id | eric_EJ1062350 |
| institution | ERIC Institute of Education Sciences |
| language | en |
| publishDate | 2015 |
| record_format | eric |
| spellingShingle | Fighting Tuberculosis in an Undergraduate Laboratory: Synthesizing, Evaluating and Analyzing Inhibitors Daniels, David Berkes, Charlotte Nekoie, Arjan Franco, Jimmy Communicable Diseases Organic Chemistry Biochemistry College Science Science Instruction Drug Therapy Undergraduate Students Science Laboratories Science Experiments Science Activities Student Projects Interdisciplinary Approach Computer Uses in Education Laboratory Experiments Fighting Tuberculosis in an Undergraduate Laboratory: Synthesizing, Evaluating and Analyzing Inhibitors Daniels, David Berkes, Charlotte Nekoie, Arjan Franco, Jimmy Communicable Diseases Organic Chemistry Biochemistry College Science Science Instruction Drug Therapy Undergraduate Students Science Laboratories Science Experiments Science Activities Student Projects Interdisciplinary Approach Computer Uses in Education Laboratory Experiments A drug discovery project has been successfully implemented in a first-year general, organic, and biochemistry (GOB) health science course and second-year organic undergraduate chemistry course. This project allows students to apply the fundamental principles of chemistry and biology to a problem of medical significance, practice basic laboratory skills, and understand the interdisciplinary aspect of the drug discovery platform. Students collectively synthesize a small library of antituberculosis compounds and subsequently screen them using a Kirby-Bauer disc diffusion assay for inhibitory activity against "Mycobacterium smegmatis", a known safe surrogate of "Mycobacterium" tuberculosis, the causative agent of tuberculosis. The last unit of the project involves students using PyMOL, a free computer program, to examine a cocrystal structure of the activated inhibitor-enzyme complex, allowing students to identify the molecular interactions responsible for the binding affinity. |
| title | Fighting Tuberculosis in an Undergraduate Laboratory: Synthesizing, Evaluating and Analyzing Inhibitors |
| topic | Communicable Diseases Organic Chemistry Biochemistry College Science Science Instruction Drug Therapy Undergraduate Students Science Laboratories Science Experiments Science Activities Student Projects Interdisciplinary Approach Computer Uses in Education Laboratory Experiments |
| url | https://eric.ed.gov/?id=EJ1062350 |