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| Format: | Dataset Open Access |
| Langue: | en |
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PANGAEA
2009
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| Accès en ligne: | https://doi.org/10.1594/PANGAEA.728727 |
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| _version_ | 1867171764240384000 |
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| author | Hauton, C Tyrrell, Toby Williams, J |
| author_facet | Hauton, C Tyrrell, Toby Williams, J |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | We report an investigation of the effects of increases in pCO2 on the survival, growth and molecular physiology of the neritic amphipod Gammarus locusta which has a cosmopolitan distribution in estuaries. Amphipods were reared from juvenile to mature adult in laboratory microcosms at three different levels of pH in nominal range 8.1-7.6. Growth rate was estimated from weekly measures of body length. At sexual maturity the amphipods were sacrificed and assayed for changes in the expression of genes coding for a heat shock protein (hsp70 gene) and the metabolic enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh gene). The data show that the growth and survival of this species is not significantly impacted by a decrease in sea water pH of up to 0.5 units. Quantitative real-time PCR analysis indicated that there was no significant effect of growth in acidified sea water on the sustained expression of the hsp70 gene. There was a consistent and significant increase in the expression of the gapdh gene at a pH of ~7.5 which, when combined with observations from other workers, suggests that metabolic changes may occur in response to acidification. It is concluded that sensitive assays of tissue physiology and molecular biology should be routinely employed in future studies of the impacts of sea water acidification as subtle effects on the physiology and metabolism of coastal marine species may be overlooked in conventional gross "end-point" studies of organism growth or mortality. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_728727 |
| institution | PANGAEA |
| language | en |
| publishDate | 2009 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Seawater carbonate chemistry and processes during experiments with amphipod Gammarus locusta, 2009 Hauton, C Tyrrell, Toby Williams, J Alkalinity, total; Animalia; Aragonite saturation state; Arthropoda; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Conductivity meter (WTW, Weilheim, Gemany); EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gammarus locusta; Gammarus locusta, survival; Hauton_etal_09; Laboratory experiment; Light:Dark cycle; Measured; Mortality/Survival; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, total scale; Salinity; SevenMulti pH meter (Mettler, Schwerzenbach, Switzerland); Single species; Temperate; Temperature, water; VINIDTA 3C instrument (Miranda, Kiel, Germany) We report an investigation of the effects of increases in pCO2 on the survival, growth and molecular physiology of the neritic amphipod Gammarus locusta which has a cosmopolitan distribution in estuaries. Amphipods were reared from juvenile to mature adult in laboratory microcosms at three different levels of pH in nominal range 8.1-7.6. Growth rate was estimated from weekly measures of body length. At sexual maturity the amphipods were sacrificed and assayed for changes in the expression of genes coding for a heat shock protein (hsp70 gene) and the metabolic enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh gene). The data show that the growth and survival of this species is not significantly impacted by a decrease in sea water pH of up to 0.5 units. Quantitative real-time PCR analysis indicated that there was no significant effect of growth in acidified sea water on the sustained expression of the hsp70 gene. There was a consistent and significant increase in the expression of the gapdh gene at a pH of ~7.5 which, when combined with observations from other workers, suggests that metabolic changes may occur in response to acidification. It is concluded that sensitive assays of tissue physiology and molecular biology should be routinely employed in future studies of the impacts of sea water acidification as subtle effects on the physiology and metabolism of coastal marine species may be overlooked in conventional gross "end-point" studies of organism growth or mortality. |
| title | Seawater carbonate chemistry and processes during experiments with amphipod Gammarus locusta, 2009 |
| topic | Alkalinity, total; Animalia; Aragonite saturation state; Arthropoda; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Conductivity meter (WTW, Weilheim, Gemany); EPOCA; EUR-OCEANS; European network of excellence for Ocean Ecosystems Analysis; European Project on Ocean Acidification; Experiment day; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Gammarus locusta; Gammarus locusta, survival; Hauton_etal_09; Laboratory experiment; Light:Dark cycle; Measured; Mortality/Survival; North Atlantic; OA-ICC; Ocean acidification; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, total scale; Salinity; SevenMulti pH meter (Mettler, Schwerzenbach, Switzerland); Single species; Temperate; Temperature, water; VINIDTA 3C instrument (Miranda, Kiel, Germany) |
| url | https://doi.org/10.1594/PANGAEA.728727 |