Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea
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| Format: | Dataset Open Access |
| Sprache: | en |
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PANGAEA
1991
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| _version_ | 1867167664058662912 |
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| author | Tan, Tjhing Lok Rüger, Hans-Jürgen |
| author_facet | Tan, Tjhing Lok Rüger, Hans-Jürgen |
| collection | Datos científicos de ciencias marinas y ambientales |
| contents | During the 'Polarstern' expedition ARK-IV/2 in June 1987, water samples from 8 stations were taken to study biomass and substrate utilization of cold adapted bacteria. Bacterial biomasses determined from acridine orange direct counts (AODC) were between 0.4 and 31.4 µ/g C/l, and ATP concentrations amounted from <0.1 to 40 ng/l. Colony counts on seawater agar reached only 0.1% of AODC, but with the MPN-method 1 to 10% of AODC were recorded. With 14C-glutamic acid or 14C-glucose as tracer substrate in oligotrophic broth containing 0.5 mg trypticase and 0.05 mg yeast extract per liter of seawater, obligately oligotrophic bacteria could be detected in one water sample. Although incubation was at 2 °C, only psychrotrophic bacteria showing growth temperatures between 1 and 30 °C were obtained. Organic substrate utilizations by 106 isolates were tested at 4 and 20 °C. Most carbohydrates, organic acids, alcohols, and alanine were assimilated at both temperatures, but arginine, aspartate and ornithine were utilized only at 20 °C by almost all strains. |
| format | Dataset Open Access |
| id | pangaea_https___doi_org_10_1594_PANGAEA_743935 |
| institution | PANGAEA |
| language | en |
| publishDate | 1991 |
| publisher | PANGAEA |
| record_format | pangaea |
| spellingShingle | Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea Tan, Tjhing Lok Rüger, Hans-Jürgen ARK-IV/2; CTD/Rosette; CTD-RO; North Greenland Sea; Polarstern; PS11; PS11/185-1; PS11/195-1; PS11/223-1; PS11/227-1; PS11/235-1; PS11/242-1; PS11/248-1; PS11/253-1 During the 'Polarstern' expedition ARK-IV/2 in June 1987, water samples from 8 stations were taken to study biomass and substrate utilization of cold adapted bacteria. Bacterial biomasses determined from acridine orange direct counts (AODC) were between 0.4 and 31.4 µ/g C/l, and ATP concentrations amounted from <0.1 to 40 ng/l. Colony counts on seawater agar reached only 0.1% of AODC, but with the MPN-method 1 to 10% of AODC were recorded. With 14C-glutamic acid or 14C-glucose as tracer substrate in oligotrophic broth containing 0.5 mg trypticase and 0.05 mg yeast extract per liter of seawater, obligately oligotrophic bacteria could be detected in one water sample. Although incubation was at 2 °C, only psychrotrophic bacteria showing growth temperatures between 1 and 30 °C were obtained. Organic substrate utilizations by 106 isolates were tested at 4 and 20 °C. Most carbohydrates, organic acids, alcohols, and alanine were assimilated at both temperatures, but arginine, aspartate and ornithine were utilized only at 20 °C by almost all strains. |
| title | Biomass and substrate utilization of cold adapted bacteria in Fram Strait and the Western Greenlad Sea |
| topic | ARK-IV/2; CTD/Rosette; CTD-RO; North Greenland Sea; Polarstern; PS11; PS11/185-1; PS11/195-1; PS11/223-1; PS11/227-1; PS11/235-1; PS11/242-1; PS11/248-1; PS11/253-1 |
| url | https://doi.org/10.1594/PANGAEA.743935 |